翼状胬肉中核糖体S6蛋白磷酸化、细胞周期蛋白D1的表达及意义

发布时间：2018-05-21 16:36

本文选题：雷帕霉素靶蛋白复合物 + 翼状胬肉　；参考：《眼科新进展》2015年04期

【摘要】：目的研究核糖体S6蛋白磷酸化(ribosomal S6 protein phosphorylation,P-S6)和细胞周期蛋白D1(CyclinD1)在翼状胬肉中的表达及相关性,探讨哺乳动物雷帕霉素靶蛋白复合物1(mammalian target of rapamycin complex 1,mTORC1)信号通路在翼状胬肉发病机制中的作用。方法收集翼状胬肉组织31例,正常结膜组织17例,利用免疫组织化学和Western blot进行P-S6和CyclinD1的检测及比较。结果 Western blot检测6例翼状胬肉组织中P-S6蛋白/S6蛋白表达(1.196±0.101)显著高于正常结膜组织(0.295±0.056),差异有统计学意义(P0.05)。免疫组织化学染色结果显示:翼状胬肉中P-S6、CyclinD1的阳性表达率均为100%(25/25),正常结膜组织中P-S6阳性表达率为18.2%(2/11)、CyclinD1阳性表达率为9.1%(1/11),正常结膜组织与翼状胬肉组织中P-S6与CyclinD1表达差异均有统计学意义(均为P.05)。翼状胬肉组织中P-S6与CyclinD1的表达呈正相关(r=0.752,P0.05)。结论 mTORC1信号通路在翼状胬肉发病过程中起重要作用,并可能通过调控CyclinD1的表达来实现。
[Abstract]:Objective to investigate the expression of ribosomal S6 protein phosphorylated S6 and cyclin D1 in pterygium, and to investigate the correlation between phosphorylation of ribosomal S6 and cyclin D1 in pterygium. To investigate the role of mammalian rapamycin target protein complex 1(mammalian target of rapamycin complex 1 mTORC1 signal pathway in the pathogenesis of pterygium. Methods 31 cases of pterygium and 17 cases of normal conjunctiva were collected. P-S6 and CyclinD1 were detected by immunohistochemistry and Western blot. Results the expression of P-S6 protein / S6 protein in 6 pterygium tissues by Western blot was significantly higher than that in normal conjunctiva tissue (P 0.05). The results of immunohistochemical staining showed that the positive rate of P-S6 cyclin D1 in pterygium was 100% 25 / 25%, the positive rate of P-S6 in normal conjunctiva was 18.2%, the positive rate of CyclinD1 was 9.1% and the expression rate of CyclinD1 in normal conjunctival tissue and pterygium tissue was lower than that in normal conjunctiva tissue and pterygium tissue, and the positive rate of P-S6 and CyclinD1 in normal conjunctiva and pterygium tissue was lower than that in normal conjunctival tissue and pterygium tissue. The difference was statistically significant (P. 05). There was a positive correlation between the expression of P-S6 and CyclinD1 in pterygium tissue. Conclusion mTORC1 signaling pathway plays an important role in the pathogenesis of pterygium and may be realized by regulating the expression of CyclinD1.
【作者单位】：南方医科大学第三附属医院眼科;
【分类号】：R777.33

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