PLUNC蛋白和TLR-4在鼻息肉组织中的表达及相关性研究

发布时间：2018-05-24 00:11

本文选题：鼻息肉 + TLR-4　；参考：《山西医科大学》2014年硕士论文

【摘要】：【目的】旨在研究PLUNC蛋白和TLR-4在鼻息肉和正常鼻粘膜组织中的表达情况及存在的差异性，分析PLUNC蛋白和TLR-4在鼻息肉组织中表达的相关性，进一步探讨二者在鼻息肉病因学及疾病进展中的意义，为研究CRSwNP的发病机制提供科学依据，奠定良好的实验基础，同时也为CRSwNP的临床治疗提供新思路。【方法】本实验标本主要收集自2013年03月~2013年11月在山西医科大学附属汾阳医院耳鼻咽喉科的住院患者，分鼻息肉组和对照组两组，术中取CRSwNP患者筛窦内息肉组织标本45例作为鼻息肉组，鼻中隔偏曲患者正常中鼻甲粘膜组织标本30例作为对照组。实验主要采用western印迹法，半定量检测正常鼻粘膜组织和鼻息肉组织中PLUNC蛋白和TLR-4的表达情况。所有实验数据均采用SPSS17.0统计软件包进行统计学分析。【结果】①PLUNC蛋白与TLR-4在鼻息肉组织与正常鼻粘膜组织中均有表达，PLUNC蛋白在鼻息肉组与正常对照组中的相对积分光密度值分别为0.054±0.022、0.535±0.266，TLR-4在两组中的相对积分光密度值分别为0.571±0.144、0.080±0.078。②PLUNC蛋白和TLR-4在鼻息肉组与对照组鼻粘膜组织的表达均存在差异且差异有统计学意义(p0.05)，TLR-4在鼻息肉组织中表达高于对照组，而PLUNC蛋白在鼻息肉组织中的表达则低于对照组；③鼻息肉组织中PLUNC蛋白与TLR-4表达存在负相关性（r=-0.724，p0.05）。【结论】①PLUNC蛋白在鼻息肉组织中低表达，而在正常鼻粘膜中高表达，表明了具有抗炎杀菌活性的PLUNC蛋白表达受限可能是导致鼻息肉发生及发展的又一重要因素。②鼻息肉组织中TLR-4表达高于正常对照组，提示感染性因素可能在鼻息肉的发病过程中起着重要的作用。TLR-4高表达可能促进了鼻息肉的发生及发展。③鼻息肉组织中PLUNC蛋白和TLR-4表达存在负相关性，提示TLR-4可能通过启动相应的信号转导通路，同时产生炎症介质、细胞因子，，从而直接抑制PLUNC蛋白的表达，造成PLUNC蛋白的产生及分泌下降。
[Abstract]:[objective] to study the expression and difference of PLUNC protein and TLR-4 in nasal polyps and normal nasal mucosa, and to analyze the correlation between PLUNC protein and TLR-4 expression in nasal polyps. To further explore the significance of the two in the etiology and disease progression of nasal polyps, to provide a scientific basis for the study of the pathogenesis of CRSwNP, to lay a good experimental foundation, and to provide new ideas for the clinical treatment of CRSwNP. [methods] the patients in the Department of Otolaryngology, Fenyang Hospital affiliated to Shanxi Medical University from March 2013 to November 2013 were collected and divided into two groups: nasal polyp group and control group. During the operation, 45 cases of polyps in ethmoid sinus of CRSwNP patients were taken as nasal polyps group, 30 cases of normal middle turbinate mucosa of patients with deviation of nasal septum as control group. The expression of PLUNC protein and TLR-4 in normal nasal mucosa and nasal polyps was detected by western blot. All experimental data were statistically analyzed by SPSS17.0 statistical software package. [results] the relative integral optical density of 1PLUNC and TLR-4 in nasal polyps and normal nasal mucosa were 0.054 卤0.022, 0.535 卤0.266, respectively. The expression of TLR-4 protein and TLR-4 in nasal mucosa of nasal polyp group and control group were significantly higher than that in nasal polyp tissue, and the expression of TLR-4 in nasal polyp tissue was significantly higher than that in control group, and the expression of TLR-4 in nasal polyp tissue was significantly higher than that in control group. However, the expression of PLUNC protein in nasal polyps was lower than that in control group (P < 0.05). There was a negative correlation between PLUNC protein and TLR-4 expression in nasal polyps. [conclusion] the expression of 1PLUNC protein is low in nasal polyps, but high in normal nasal mucosa. The results suggested that the limited expression of PLUNC protein with anti-inflammatory bactericidal activity might be another important factor leading to the occurrence and development of nasal polyps. 2 the expression of TLR-4 in nasal polyps was higher than that in normal controls. The results suggest that infectious factors may play an important role in the pathogenesis of nasal polyps. The high expression of TLR-4 may promote the occurrence and development of nasal polyps. 3. There is a negative correlation between PLUNC protein and TLR-4 expression in nasal polyps. It is suggested that TLR-4 may directly inhibit the expression of PLUNC protein and decrease the production and secretion of PLUNC protein by activating the corresponding signal transduction pathway and simultaneously producing inflammatory mediators and cytokines.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R765.25

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