HSP70自身抗体和EB病毒VCA-IgA联合检测对鼻咽癌诊断价值的探讨

发布时间：2018-05-25 00:40

本文选题：热休克蛋白 + 自身抗体　；参考：《中华肿瘤防治杂志》2015年15期

【摘要】：目的探讨热休克蛋白(heat shock protein,HSP)70自身抗体和EB病毒衣壳抗原(viral capsid antigen,VCA)-IgA抗体联合检测对鼻咽癌(nasopharyngeal carcinoma,NPC)的诊断意义。方法收集2012-05-03-2014-04-22汕头大学医学院附属肿瘤医院收治的209例NPC患者和216名正常对照,采用酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)检测血清中HSP70自身抗体和EB病毒VCA-IgA的表达水平。结果 NPC患者血清HSP70自身抗体的表达水平明显高于正常对照,P0.001。HSP70自身抗体对NPC的诊断敏感度为28.7%,95%CI为22.8~35.4;VCA-IgA为49.3%,95%CI为42.3~56.2。HSP70自身抗体诊断的特异度为94.9%,95%CI为90.8~97.3;VCA-IgA为95.8%,95%CI为92.0~98.0。两者联合检测的敏感度为63.2%,95%CI为56.2~69.6;特异度为91.2%,95%CI为86.4~94.5。两者联合检测的阳性率在早期NPC患者为53.1%(95%CI为35.0~70.5),高于正常对照的8.8%(95%CI为5.5~13.6),P0.001。HSP70自身抗体的阳性率与NPC患者的性别相关,P0.05;而与患者年龄、T分期、N分期和总TNM分期无关,P0.05。结论血清HSP70自身抗体对NPC具有潜在的诊断价值,与VCA-IgA联合检测可能有助于提高NPC的早期诊断率。
[Abstract]:Objective to investigate the diagnostic significance of combined detection of heat shock protein (HSP) 70 autoantibody and Epstein-Barr virus (EBV) capsid antigen viral capsid antigen-IgA antibody in nasopharyngeal carcinoma (NPC). Methods 209 patients with NPC and 216 normal controls were collected from tumor Hospital affiliated to Shantou University Medical College from 2012-05-03-2014-04-22. Serum HSP70 autoantibodies and EB virus VCA-IgA were detected by enzyme-linked immunosorbent assay-ELISA-linked enzyme linked immunosorbent assay (Elisa). Results the serum HSP70 autoantibody expression level in NPC patients was significantly higher than that in normal controls (P 0.001. HSP70 autoantibody). The diagnostic sensitivity of serum HSP70 autoantibodies to NPC was 28.70.95 CI = 22.80.35. 4 and VCA-IgA was 49.30.95 CI, the diagnostic specificity of 42.3~56.2.HSP70 autoantibodies was 94.99.97. 3VCA-IgA was 90.897.3VCA-IgA and 95.89.95CI was 92.00.98. 0. The sensitivity of the combined detection was 63.2 and 95 CI was 56.2 and 69.6 respectively, and the specificity was 91.2 and the CI was 86.4 / 94.5. The positive rate of 53.1%(95%CI in early NPC patients was 35.0 / 70.5g, which was higher than that in normal controls (8.8%(95%CI = 5.5 / 13.6). The positive rate of autoantibodies to HSP70 was correlated with the sex of NPC patients (P0.05), but had no correlation with the age of patients with T staging and total TNM stage (P0.05). Conclusion Serum HSP70 autoantibodies have potential diagnostic value for NPC, and combined detection with VCA-IgA may be helpful to improve the early diagnosis rate of NPC.
【作者单位】：汕头大学医学院附属肿瘤医院检验科;汕头大学医学院附属肿瘤医院头颈外科;汕头大学医学院附属肿瘤医院放疗科;
【基金】：广东省科技计划(2013B021800250) 汕头大学医学院临床研究提升计划(201428)
【分类号】：R739.63;R730.43

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