应用眼眶淋巴瘤组织芯片检测P-gp、GST-π及LRP的表达及其临床意义

发布时间：2018-05-27 18:36

  本文选题：眼眶淋巴瘤 + P-gp　； 参考：《天津医科大学》2011年硕士论文

【摘要】：研究目的 制作眼眶淋巴瘤组织芯片；利用免疫组织化学检测三种耐药蛋白在眼眶淋巴瘤中的表达情况,初步探索该病的复发及预后不良机制 研究方法 1收集2003年至2008年期间诊断为眼眶淋巴瘤及反应性淋巴增生患者的临床病理资料,并对病人进行随访。 2制作眼眶淋巴瘤组织芯片。 3应用免疫组织化学检测P-gp、GST-π、LRP在眼眶淋巴瘤中的表达。 4应用SPSS13.0统计学软件对数据进行分析,以α=0.05为检验水准,P0.05认为有统计学意义。 研究结果 1 50例眼眶淋巴瘤和17例眼眶反应性淋巴增生组织芯片经HE染色后,病理诊断结果与原取材的大组织诊断完全一致；有4例眼眶淋巴瘤在免疫组织化学的染色过程中一脱片,因此末对这4例标本的染色结果进行统计。 2三种耐药蛋白,P-gp和GST-π在眼眶淋巴瘤和反应性淋巴增生组织芯片中有不同程度表达,LRP在两种组织中均为阴性表达。P-gp蛋白主要表达于细胞膜,在两种病变的阳性表达率为87.0%和35.3%,其差异有统计学意义；GST-π表达于细胞浆,在两种病变的阳性表达率为76.1%和41.2%,其差异有统计学意义。 3 46例淋巴瘤：P-gp和GST-π这两种蛋白的阳性表达率在≥60岁和60岁年龄组间、男女性别组间、≥2.5cm和2.5cm肿瘤组间及≥1年和1年发病时间组间均无统计学意义。 4将有随访结果的35例眼眶淋巴瘤分为两组,即预后不良组和预后良好组；P-gp在两组间的表达率分别为85.7%和42.9%,其差异有统计学意义；GST-π在两组间的表达率分别为78.7%和38.1%,其差异有统计学意义。 5 P-gp和GST-π在眼眶淋巴瘤中的表达呈正相关(P0.05)。 结论 1眼眶淋巴瘤组织芯片省时快速,结果可靠,高效经济地研究了该肿瘤的多药耐药基因表达状况。 2 P-gp在眼眶淋巴瘤和反应性淋巴增生组间阳性比率表达差异有统计学意义,说明P-gp对判断眼眶淋巴细胞性疾病的病变性质有一定价值；同时P-gp在眼眶淋巴瘤预后不良组和预后良好组间阳性率表达差异有统计学意义,说明P-gp可以预测眼眶淋巴瘤的预后。 3 GST-π在眼眶淋巴瘤和反应性淋巴增生组间阳性率表达差异有统计学意义,说明P-gp对判断眼眶淋巴细胞性疾病的病理性质有一定意义；同时GST-π在眼眶淋巴瘤预后不良组和预后良好组间阳性率表达差异有统计学意义,说明GST-π可以预测眼眶淋巴瘤的预后 4 LRP在眼眶淋巴瘤和反应性淋巴增生组均呈阴性表达,提示LRP与眼眶淋巴细胞性疾病的发生发展无明确相关性。 5 P-gp和GST-π蛋白在眼眶淋巴瘤中的表达呈正相关,联合检测这两种指标可能对预测眼眶淋巴瘤预后有更大价值。
[Abstract]:Research purpose The tissue microarray of orbital lymphoma was made and the expression of three kinds of drug-resistant proteins in orbital lymphoma was detected by immunohistochemistry to explore the recurrence of the disease and the mechanism of poor prognosis. Research method 1 clinicopathological data of patients with orbital lymphoma and reactive lymphoid hyperplasia from 2003 to 2008 were collected and followed up. 2 making tissue microarray of orbital lymphoma. 3Immunohistochemistry was used to detect the expression of P-gpt- 蟺 -LRP in orbital lymphoma. 4 SPSS13.0 statistical software was used to analyze the data, and the 伪 0. 05 test level was considered to be statistically significant (P0.05). Research results 150 cases of orbital lymphoma and 17 cases of orbital reactive lymphoid hyperplasia were stained by HE staining. Therefore, the staining results of these 4 specimens were statistically analyzed. 2the expression of P-gp and GST- 蟺 in orbital lymphoma and reactive lymphoproliferative tissue microarray was negative in both tissues. P-gp protein was mainly expressed on cell membrane. The positive expression rates of GST- 蟺 in the two lesions were 87.0% and 35.3.The difference was statistically significant. The positive expression rates of GST- 蟺 were 76.1% and 41.2% in the two lesions, respectively. The difference was statistically significant. 3The positive expression rates of P-gp and GST- 蟺 proteins in 46 cases of lymphoma were not significantly different between the age group 鈮，

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