共同性外斜视内直肌IGF-Ⅰ、AnnexinA1的表达研究

发布时间：2018-05-28 03:19

  本文选题：共同性外斜视 + 胰岛素样生长因子　； 参考：《青岛大学》2011年硕士论文

【摘要】：目的观察共同性外斜视眼外肌中IGF-1、Annexin A1表达的变化,探讨IGF-1、Annexin A1在共同性外斜视发病机制中的作用。 方法收集我院2009-2010年收治的共同性外斜视病人(16例)为斜视组。根据年龄又将斜视组分为儿童斜视组(8例)和成人斜视组(8例)；同期行眼球摘除或角膜移植供体取得的水平直肌为成人对照组(共6例)。所有眼外肌固定切片后行IGF-1、Annexin A1免疫组织化学染色,测IGF-1、Annexin A1在眼外肌中的平均光密度值。对各组间平均光密度值进行比较,并对斜视患者眼外肌中IGF-1平均光密度值与患者斜视度及年龄进行相关性分析。 结果①IGF-1在斜视组、成人对照组、儿童斜视组及成人斜视组表达的平均光密度值分别为(0.225±0.041)、(0.334±0.024)、(0.255±0.012)及(0.196±0.038)。斜视组与成人对照组、儿童斜视组与成人斜视组、成人斜视组与成人对照组之间比较均有统计学意义(P0.05)。②Annexin A1平均光密度值在斜视组、成人对照组、儿童斜视组及成人斜视组表达的平均光密度值分别为(0.148±0.031)、(0.254±0.050)、(0.197±0.027)及(0.126±0.026)。斜视组与成人对照组、儿童斜视组与成人斜视组、成人斜视组与成人对照组之间比较同样均有统计学意义(P0.05)。③斜视患者IGF-1、AnnexinAl平均光密度值与患者的斜视度和年龄呈负相关(r=-0.721,P=0.002,r=-0.663,P=0.008；r=-0.554,P=0.029,r=-0.598,P=0.014)。 结论①眼外肌中IGF-1、Annexin A1表达的降低可能参与斜视的发病。②眼外肌中IGF-1、Annexin A1中的表达变化可能与斜视的发展相关。
[Abstract]:Objective to observe the expression of IGF-1 and Annexin A1 in the extraocular muscle of concomitant exotropia and to explore the role of IGF-1 and Annexin A1 in the pathogenesis of concomitant exotropia. Methods 16 patients with concomitant exotropia admitted in our hospital from 2009-2010 were selected as strabismus group. According to the age, the strabismus group was divided into children strabismus group (n = 8) and adult strabismus group (n = 8). All the sections of the extraocular muscle were fixed with the IGF-1 Annexin A1 immunohistochemical staining, and the mean optical density of the IGF-1 and Annexin A1 in the extraocular muscle was measured. The mean optical density of the patients with strabismus was compared and the correlation between the mean optical density of IGF-1 in the extraocular muscles and the degree of strabismus and age was analyzed. Results the average optical density of 1IGF-1 expression in strabismus group, adult control group, strabismus group and adult strabismus group were 0.225 卤0.041, 0.334 卤0.024, 0.255 卤0.012 and 0.196 卤0.038, respectively. There were significant differences between strabismus group and adult control group, children strabismus group and adult strabismus group, adult strabismus group and adult control group. The average optical density of squint group and adult strabismus group were 0.148 卤0.031, 0.254 卤0.050, 0.197 卤0.027) and 0.126 卤0.026, respectively. In strabismus group and adult control group, children strabismus group and adult strabismus group, adult strabismus group and adult strabismus group, there was also significant difference between the mean optical density of IGF-1 and Annexin Al in strabismus patients. There was a negative correlation between the mean optical density of IGF-1 and Annexin Al and the strabismus degree and age of the patients, and the mean optical density of IGF-1 and Annexin Al was negatively correlated with the strabismus degree and the age of the patients with strabismus, the squint group and the adult strabismus group. Conclusion 1 the decrease of the expression of IGF-1 and Annexin A1 in extraocular muscles may be involved in the pathogenesis of strabismus .2 the changes of the expression of IGF-1 and Annexin A1 in extraocular muscles may be related to the development of strabismus.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R779.6

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