HIF-1α和VEGF在喉癌中的表达及其与血管生成的关系

发布时间：2018-05-28 22:34

本文选题：喉肿瘤 + 血管形成　；参考：《临床耳鼻咽喉头颈外科杂志》2014年06期

【摘要】：目的:研究缺氧诱导因子-1α(HIF-1α)及血管内皮生长因子(VEGF)在喉癌组织中的表达程度及其与喉癌临床病理学特征之间的关系,并探讨HIF-1α及VEGF在肿瘤血管生成中的作用。为喉癌的发生机制提供理论基础,并为喉癌的早期诊断提供新思路。方法:试验分组:60例喉癌标本及20例癌旁正常组织。采用免疫组织化学方法检测HIF-1α及VEGF表达情况,并用CD105标记血管内皮细胞,计算微血管密度(MVD)。结果:60例喉癌组织中,HIF-1α、VEGF阳性表达率分别为71.67%(43/60)、65.00%(39/60),MVD平均值为33.82±10.15;20例癌旁正常组织中,HIF-1α、VEGF阳性表达率分别为10%(2/20)、20%(4/20),MVD平均值为21.48±6.84。喉癌组织中HIF-1α、VEGF阳性表达率显著高于正常喉组织。HIF-1α在喉癌组织中的阳性表达率与癌组织的临床分期、分化程度和淋巴结转移密切相关。VEGF阳性表达率与临床分期及有无淋巴结转移相关,而与组织分化程度无关。HIF-1α与VEGF表达一致符合率为56.67%(34/60),两者表达呈明显正相关。HIF-1α、VEGF均阳性的喉癌组织MVD高于两者均为阴性者,差异有统计学意义(P0.01)。结论:喉癌组织中存在HIF-1α蛋白及VEGF的过表达,HIF-1α、VEGF与MVD在喉癌中的表达呈正相关,协同促进肿瘤新血管形成。
[Abstract]:Objective: to study the expression of hypoxia inducible factor-1 伪 (HIF-1 伪) and vascular endothelial growth factor (VEGF) in laryngeal carcinoma and their relationship with the clinicopathological features of laryngeal carcinoma, and to explore the role of HIF-1 伪 and VEGF in tumor angiogenesis. It provides a theoretical basis for the pathogenesis of laryngeal carcinoma and a new idea for early diagnosis of laryngeal carcinoma. Methods: 60 laryngeal cancer specimens and 20 adjacent normal tissues were divided into two groups. The expression of HIF-1 伪 and VEGF was detected by immunohistochemical method. Vascular endothelial cells (VEC) were labeled with CD105 to calculate the microvessel density (MVD). Results the positive rates of HIF-1 伪 -VEGF expression in 60 cases of laryngeal carcinoma were 71.67 and 65.00, respectively. The average MVD of 39 / 60 ~ (-0) ~ (39) / 60 ~ (-1) was 33.82 卤10.15 ~ 20 cases of normal tissues adjacent to carcinoma. The positive rate of VEGF expression in 20 cases of normal tissues adjacent to carcinoma was 10 ~ (2 / 20)% ~ 20 ~ (-20)% ~ (4) ~ (20)% ~ (20) MVD = 21.48 卤6.84, respectively, and the mean value of MVD was 21.48 卤6.84 in 20 cases of normal tissues adjacent to carcinoma. The positive expression rate of HIF-1 伪 -VEGF in laryngeal carcinoma was significantly higher than that in normal laryngeal tissue. The degree of differentiation was closely related to lymph node metastasis. The positive expression rate of VEGF was correlated with clinical stage and lymph node metastasis. The coincident rate between HIF-1 伪 and VEGF expression was 56.670.34 / 60, and the positive correlation between HIF-1 伪 and VEGF was significantly higher in laryngeal carcinoma than that in both negative tissues (P 0.01). Conclusion: there is a positive correlation between the expression of HIF-1 伪 protein and VEGF in laryngeal carcinoma and the expression of MVD in laryngeal carcinoma.
【作者单位】：天津市滨海新区大港医院耳鼻咽喉科;天津医科大学总医院耳鼻咽喉科;
【分类号】：R739.65

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