粉防己碱对大鼠角膜新生血管中血管生成相关因子的影响

发布时间：2018-05-29 00:51

本文选题：粉防己碱 + 大鼠　；参考：《华中科技大学》2011年硕士论文

【摘要】：目的:研究粉防己碱对大鼠碱烧伤角膜新生血管中VEGF-C、TGF-β1、bFGF、IGF-1和MIP-2等5种细胞因子基因表达的影响,并探讨粉防己碱对大鼠碱烧伤角膜炎症反应及新生血管生长的影响。方法:取SD大鼠共计56只,8只作为阴性对照组,眼球不做任何处理,48只建立角膜碱烧伤诱导的角膜新生血管动物模型,左眼滴生理盐水作为对照组,右眼滴粉防己碱溶液作为实验组。造模后每天采用裂隙灯观察,比较三组角膜炎症反应及新生血管生长情况,在造模后第1、4、7和14天分别进行实时荧光定量RT-PCR检测,比较VEGF-C、TGF-β1、bFGF、IGF-1、MIP-2等5种细胞因子在三组角膜新生血管中表达的差异。结果:阴性对照组在各个时间点角膜均无炎症反应发生及新生血管生长,对照组和实验组角膜均于第1天开始出现明显炎症反应,并随时间延长逐渐加重,7天时炎症反应最重,14天时明显减轻且轻于第4天;两组均于第3天从角膜缘处发生新生血管,并随时间延长逐渐生长,10天时生长最旺盛,14天时血管生长已停滞并较10天时减少;在第1天和第7天时对照组和实验组两组之间角膜炎症反应和新生血管均无明显差异,在其它时间点实验组较对照组炎症反应轻,新生血管数量少、管径细。对照组和实验组VEGF-C mRNA表达量在各个时间点均较阴性对照组显著增多(P0.05),并随时间延长逐渐增加。实验组VEGF-C mRNA表达量在各个时间点均较对照组少,两组之间VEGF-C mRNA表达量在各个时间点差异均具有统计学意义(P0.05)。对照组和实验组TGF-β1 mRNA表达量在各个时间点均较阴性对照组显著增多(P0.05),并随时间延长逐渐下降。实验组TGF-β1 mRNA表达量在各个时间点均较对照组少,两组之间TGF-β1 mRNA表达量在各个时间点差异均具有统计学意义(P0.05)。MIP-2 mRNA表达量和TGF-β1 mRNA表达量具有相同的特征。对照组和实验组bFGF mRNA表达量在各个时间点均较阴性对照组显著增多(P0.05);对照组bFGF mRNA表达量在7天时达到高峰,14天时较7天时有所下降,而实验组bFGF mRNA表达量随时间延长逐渐增加。实验组bFGF mRNA表达量在各个时间点均较对照组多,两组之间bFGF mRNA表达量在各个时间点差异均具有统计学意义(P0.05)。对照组和实验组IGF-1 mRNA表达量在各个时间点均较阴性对照组显著增多(P0.05),并随时间延长逐渐增加,7天时达到高峰,14天时较7天时有所下降。实验组IGF-1 mRNA表达量在各个时间点均较对照组少,两组之间IGF-1 mRNA表达量在各个时间点差异均具有统计学意义(P0.05)。结论:粉防己碱可明显抑制血管生成刺激因子VEGF-C、TGF-β1、IGF-1及炎症趋化因子MIP-2的基因表达,但同时也促进血管生成刺激因子bFGF的基因表达。粉防己碱可抑制大鼠碱烧伤角膜的炎症反应及新生血管生长。
[Abstract]:Objective: to study the effect of Tetrandrine on the expression of VEGF-C1bFGF-1 and MIP-2 in corneal neovascularization after alkali burn in rats, and to investigate the effects of Tetrandrine on corneal inflammation and neovascularization in rats with alkali burn. Methods: a total of 56 SD rats were used as negative control group, 48 rats were made into corneal neovascularization model induced by alkali burn of cornea, and the left eye was treated with normal saline as control group. The right eye was treated with Tetrandrine solution. The corneal inflammation and neovascularization were observed by slit lamp every day, and real-time quantitative RT-PCR was performed on the 7th and 14th day after modeling. To compare the expression of five cytokines such as TGF- 尾 1bFGF-1 and MIP-2 in corneal neovascularization in three groups. Results: there was no inflammatory reaction and neovascularization in the cornea of the negative control group at all time points. The corneal inflammation in both the control group and the experimental group began to appear on the first day. The inflammatory response decreased significantly at day 14 and was lighter than that on day 4, and neovascularization occurred at day 3 from the limbus of cornea in both groups. At the 14th day, the blood vessel growth was stopped and decreased compared with the 10th day, and there was no significant difference in corneal inflammation and neovascularization between the control group and the experimental group on the 1st and 7th day, and there was no significant difference between the control group and the experimental group in corneal inflammation and neovascularization. At other time points, the inflammatory reaction in the experimental group was lighter than that in the control group, the number of neovascularization and the diameter of the vessel were smaller. The expression of VEGF-C mRNA in the control group and the experimental group was significantly higher than that in the negative control group at each time point, and gradually increased with time. The expression of VEGF-C mRNA in the experimental group was lower than that in the control group at each time point, and the difference of VEGF-C mRNA expression between the two groups at each time point was statistically significant (P 0.05). The expression of TGF- 尾 1 mRNA in the control group and the experimental group was significantly higher than that in the negative control group at all time points, and decreased gradually with time. The expression of TGF- 尾 1 mRNA in the experimental group was less than that in the control group at each time point, and the difference of TGF- 尾 1 mRNA expression between the two groups at each time point was statistically significant. The expression of TGF- 尾 1 mRNA and TGF- 尾 1 mRNA had the same characteristics. The expression of bFGF mRNA in the control group and the experimental group was significantly higher than that in the negative control group at each time point, while the expression of bFGF mRNA in the control group reached the peak at the 7th day and decreased at the 14th day, while the bFGF mRNA expression in the experimental group gradually increased with the extension of time. The expression of bFGF mRNA in the experimental group was higher than that in the control group at each time point, and the difference of bFGF mRNA expression between the two groups at each time point was statistically significant (P 0.05). The expression of IGF-1 mRNA in the control group and the experimental group was significantly higher than that in the negative control group at each time point, and gradually increased at 7 days and reached the peak at 14 days and decreased at the same time. The expression of IGF-1 mRNA in the experimental group was lower than that in the control group at each time point, and the difference of IGF-1 mRNA expression between the two groups at each time point was statistically significant (P 0.05). Conclusion: Tetrandrine can significantly inhibit the gene expression of VEGF-CnTGF- 尾 1 and inflammatory chemokine MIP-2, but also promote the gene expression of angiogenic stimulating factor bFGF. Tetrandrine can inhibit corneal inflammation and neovascularization in rats with alkali burn.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R285.5;R779.1

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