CTGF反义寡核苷酸对体外培养人晶状体上皮细胞转分化的影响

发布时间：2018-05-30 01:31

本文选题：结缔组织生长因子 + 晶状体上皮细胞　；参考：《海峡科学》2010年05期

【摘要】：目的:研究结缔组织生长因子(connective tissue growth factor,CTGF)反义寡核苷酸对体外培养人晶状体上皮细胞(human lens epithelial cells,HLECs)合成CTGF与а-SMA表达的影响。方法:测算CTGF反义寡核苷酸对体外培养HLECs的转染率;应用CTGF反义寡核苷酸直接转染第三代HLECs进行细胞生长曲线的测定;采用RT-PCR检测细胞CTGF和α-平滑肌动蛋白(α-smooth muscle actin,α-SMA)mRNA水平。结果:直接导入法反义寡核苷酸进入较慢,但导入72h后未出现细胞毒性。CTGF反义寡核苷酸直接导入HLECs培养细胞72h后可见对细胞增殖的抑制作用。直接导入法处理48h后,TGF-β1能显著增加CTGF以及α-SMA mRNA的表达,但却可被CTGF反义寡核苷酸所抑制。而错义寡核苷酸却没有这种作用。结论:CTGF反义寡核苷酸直接转染HLECs能抑制TGF-β1诱导的CTGF与α-SMA表达上调,提示CTGF反义寡核苷酸可能为防治后发性白内障提供新途径。
[Abstract]:Objective: To study the effect of connective tissue growth factor (CTGF) antisense oligonucleotides on the synthesis of CTGF and -SMA expression in cultured human lens epithelial cells (human lens epithelial cells, HLECs) in vitro. The cell growth curve was measured by direct transfection of third generations of HLECs; RT-PCR was used to detect CTGF and alpha smooth actin (alpha -smooth muscle actin, alpha -SMA) mRNA level. Results: direct introduction of antisense oligodeoxynucleotides was slow, but no cytotoxic.CTGF antisense oligodeoxynucleotides were directly introduced into HLECs culture cell 72h after the introduction of 72h After direct introduction of 48h, TGF- beta 1 can significantly increase the expression of CTGF and alpha -SMA mRNA, but can be suppressed by CTGF antisense oligonucleotides. But missense oligonucleotides have no such effect. Conclusion: CTGF antisense oligonucleotides directly transfected HLECs can inhibit the expression of TGF- beta 1 induced CTGF and alpha -SMA. Up regulation suggests that CTGF antisense oligonucleotides may provide a new way to prevent and treat posterior capsule opacification.
【作者单位】：福建医科大学附属第一医院福建省眼科研究所;
【基金】：国家自然科学基金重点项目(基金编号:60827002) 福建医科大学教授发展基金课题(基金编号:2006-js6033)
【分类号】：R776.1;R779.66

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