清瘤亮喉方对喉咽癌移植瘤生物学行为的影响及其机理研究

发布时间：2018-06-01 17:01

本文选题：喉咽癌 + 梨状窝　；参考：《山东中医药大学》2011年博士论文

【摘要】：目的：研究清瘤亮喉方对喉咽癌移植瘤生物学行为的影响,并探讨其治疗或干预喉咽癌的作用机制。方法：1.建立梨状窝兔VX2瘤模型兔：VX2瘤细胞株荷瘤新西兰大白兔传代,以传代兔肿瘤组织块,制成肿瘤组织液,直达喉镜下接种于兔梨状窝。2.研究清瘤亮喉方对喉咽癌移植瘤生物学行为的影响：新西兰大白兔46只接种肿瘤后,随机分为空白组、对照组(氟尿嘧啶组)、治疗组(清瘤亮喉方组),除7只种植兔意外死亡外,余39只进入研究。随后观察其生存情况、影像学(CT)、解剖学、病理学(HE染色)及免疫组化(细胞周期调控因子cyclinB、cyclinD、CDK4及P27)研究。结果：服用清瘤亮喉方组(治疗组)及氟尿嘧啶组(对照组)的梨状窝VX2瘤兔的生存质量及存活时间明显优于空白组(p0.05)；治疗组与对照组的梨状窝VX2瘤兔的肿瘤生长情况在解剖及影像学上与空白组存在显著差异(p0.05)；治疗组与对照组的梨状窝VX2瘤兔的肿瘤生长情况在病理学上与空白组存在显著差异(p0.05)；治疗组与对照组、空白组的梨状窝VX2瘤兔的肿瘤生长情况在免疫组化研究上存在显著差异(p0.05)。结论：1.清瘤亮喉方能有效改善梨状窝VX2瘤兔的生存质量,延长肿瘤兔的生命。2.清瘤亮喉方能干预喉咽癌移植瘤的生长,其机制可能是通过诱导肿瘤细胞凋亡。3.清瘤亮喉方对喉咽癌的治疗作用,体现了中药对机体免疫功能的调节。
[Abstract]:Objective: to study the effect of Qinglianghou recipe on the biological behavior of transplanted laryngopharyngeal carcinoma, and to explore the mechanism of its treatment or intervention in laryngopharyngeal carcinoma. Method 1: 1. The rabbit VX2 tumor model of piriform nest rabbit was established. The rabbit VX2 tumor cell line was subcultured into rabbit tumor tissue fluid and inoculated directly into rabbit piriform fossa under laryngoscope. To study the effect of Qinglianghou recipe on the biological behavior of transplanted laryngopharyngeal carcinoma, 46 New Zealand white rabbits were randomly divided into blank group after inoculation of tumor. The control group (fluorouracil group), the treatment group (Qingliulianghou prescription group), except 7 implanted rabbits died accidentally, the other 39 entered the study. Then, the survival status, imaging CTU, anatomy, pathology and HE staining were observed, and immunohistochemistry (cyclinBon cyclin DCDK4 and P27) were studied. Results: the quality of life and survival time of piriform fossa VX2 tumor rabbits in the treatment group and fluorouracil group were significantly better than those in the blank group (P 0.05), and the tumorigenesis of the piriform fossa VX2 tumor rabbits in the treatment group and the control group was significantly better than that in the control group. There were significant differences in anatomy and imaging between the treatment group and the blank group (p 0.05A), the growth of the VX2 tumor in the piriform fossa between the treatment group and the control group was significantly different from that in the blank group (P 0.05), and the tumor growth in the treatment group and the control group was significantly different from that in the blank group. There was a significant difference in tumor growth of piriform fossa VX2 tumor rabbits in the blank group in immunohistochemical study. Conclusion 1. Qingliangyao prescription can effectively improve the quality of life of VX2 tumor rabbits in piriform fossa and prolong the life of tumor rabbits. Qinglianghou prescription can interfere with the growth of transplanted tumor of laryngopharyngeal carcinoma, and its mechanism may be by inducing apoptosis of tumor cells. 3. The therapeutic effect of Qinglianghou prescription on laryngopharynx carcinoma reflects the regulation of immune function of traditional Chinese medicine.
【学位授予单位】：山东中医药大学
【学位级别】：博士
【学位授予年份】：2011
【分类号】：R739.65

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