轴突导向因子-1在氧诱导视网膜病变小鼠视网膜中的表达

发布时间：2018-06-01 21:32

本文选题：轴突导向因子-1 + 氧诱导视网膜病变　；参考：《中南大学》2010年硕士论文

【摘要】： 目的：探讨Netrin-1蛋白在C57BL/6J小鼠氧诱导视网膜病变(OIR)模型中视网膜中的表达,及其与视网膜新生血管形成的相关性。方法：随机取16只C57BL/6J新生小鼠分为正常组和高氧诱导组,每组8只。通过高氧诱导的方法使用C57BL/6J小鼠建立氧诱导视网膜病变(OIR)模型。小鼠17日龄时,先于各组随机取出2只小鼠荧光造影后行视网膜铺片,通过观察视网膜新生血管的形态来鉴定视网膜新生血管模型；剩余小鼠全部处死后取出眼球固定,行冰冻切片,免疫组织化学技术检测Netrin-1蛋白质在视网膜中的表达。结果：(1)荧光造影视网膜铺片观察视网膜血管形态：正常组17日龄小鼠视网膜铺片显示视网膜血管分布呈均匀的网状结构。OIR模型组17日龄小鼠视网膜视盘周边见大片无灌注区。无灌注区周围可见新生血管,伴明显荧光渗漏。(2)免疫荧光结果；OIR模型小鼠建立成功,17日龄的OIR模型组小鼠视网膜处于新生血管增生期,视网膜浅层可见Netrin-1蛋白明显表达,而17日龄正常组小鼠视网膜未见Netrin-1蛋白表达。结论：OIR小鼠视网膜新生血管期Netrin-1在视网膜浅层表达,提示Netrin-1可能参与视网膜新生血管的形成过程。
[Abstract]:Aim: to investigate the expression of Netrin-1 protein in the retina of C57BL/6J mice induced by oxygen induced retinopathy and its correlation with retinal neovascularization. Methods: sixteen C57BL/6J newborn mice were randomly divided into normal group and hyperoxia induced group with 8 mice in each group. The model of oxygen-induced retinopathy was established in C57BL/6J mice by hyperoxia induction. At the age of 17 days, the retinal neovascularization model was identified by observing the morphology of retinal neovascularization, and the rest of the mice were killed and their eyeballs were fixed. The expression of Netrin-1 protein in retina was detected by immunohistochemistry. Results the retinal vascular morphology was observed by fluorescein angiography (FG): the retinal vascular distribution was uniform in the normal group of 17 days old mice. In the oir model group, there was a large area of no perfusion around the optic disc of the 17 day old mouse retina. Neovascularization was seen around the no perfusion area, accompanied by obvious fluorescence leakage. (2) the results of immunofluorescence showed that the OIR model mice of 17 days old were in the phase of neovascularization, and the Netrin-1 protein was obviously expressed in the superficial layer of the retina. However, no expression of Netrin-1 protein was found in the retina of 17-day-old normal mice. Conclusion the expression of Netrin-1 in retinal neovascularization phase of OIR mice in the superficial layer of retina suggests that Netrin-1 may be involved in the formation of retinal neovascularization.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R774.1

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