MKK4基因在喉肿瘤中的表达及其临床意义

发布时间：2018-06-01 21:41

  本文选题：喉肿瘤 + 反转录-聚合酶链反应　； 参考：《重庆医科大学》2010年硕士论文

【摘要】： 目的:研究MKK4基因在喉肿瘤中的表达,探讨它们的临床意义及相互关系,为喉癌的发生发展、预后评估及寻求新的基因治疗提供实验依据。 方法:运用反转录-聚合酶链反应(RT-PCR)和免疫组织化学方法检测42例喉鳞癌、20例喉乳头状瘤及20例声带息肉组织中MKK4 mRNA和蛋白的表达,分析它们在不同病变组织中的表达情况及与喉鳞癌临床病理参数的关系。 结果: 1.MKK4 mRNA和蛋白表达的阳性率在喉鳞癌、喉乳头状瘤及声带息肉组织中逐渐降低,其差异有统计学意义(P0.05)。 2.MKK4 mRNA和蛋白的表达随喉鳞癌的浸润深度增加而增加(P0.05),在淋巴结转移组高于无淋巴结转移组(P0.05),在临床Ⅲ~Ⅳ期的表达高于临床Ⅰ~Ⅱ的表达(P0.05)。 3.MKK4表达率与患者年龄、性别、肿瘤发生部位及组织学分级无相关性(P0.05)。 结论: 1.MKK4 mRNA和蛋白在喉鳞癌组织中的上调可能与喉鳞癌的发生及浸润转移有关。 2.MKK4可能作为一个潜在的判断喉鳞癌发病与转移的观测指标。
[Abstract]:Objective: to study the expression of MKK4 gene in laryngeal neoplasms, and to explore their clinical significance and relationship, so as to provide experimental evidence for the occurrence and development of laryngeal carcinoma, prognosis evaluation and search for new gene therapy. Methods: reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to detect the expression of MKK4 mRNA and protein in 20 cases of laryngeal papilloma and 20 cases of vocal cord polyp. To analyze their expression in different pathological tissues and their relationship with the clinicopathologic parameters of laryngeal squamous cell carcinoma (LSCC). Results: The positive rates of 1.MKK4 mRNA and protein in laryngeal squamous cell carcinoma, laryngeal papilloma and vocal cord polyp decreased gradually, and the difference was statistically significant (P 0.05). The expression of 2.MKK4 mRNA and protein increased with the depth of invasion of laryngeal squamous cell carcinoma. The expression of 2.MKK4 mRNA and protein in lymph node metastasis group was higher than that in non-lymph node metastasis group, and the expression in clinical stage 鈪，

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