核转录因子-κB在氧诱导血管增殖性视网膜病变小鼠中的表达

发布时间：2018-06-02 04:37

本文选题：核转录因子-κB + 氧诱导　；参考：《眼科新进展》2014年12期

【摘要】：目的探讨氧诱导血管增殖性视网膜病变小鼠视网膜中核转录因子(nuclear factor,NF)-κB蛋白的表达变化及意义。方法 72只出生后7 d的C57BL/6J小鼠随机分为实验组(36只)和对照组(36只),实验组与母鼠一起放入氧气含量为体积分数75%的饲养箱中饲养,出生后12 d回到正常大气环境中;对照组小鼠始终在正常大气环境中饲养。分别于出生后12 d、14 d、17 d时处死小鼠,荧光素灌注后行视网膜铺片观察血管分布及形态,Western blot法检测视网膜中血管内皮生长因子(vascular endothelial growth factor,VEGF)的蛋白表达变化,免疫组织化学染色检测眼球中NF-κB的表达变化。结果视网膜铺片检查结果可见,实验组小鼠出生后14 d开始出现视网膜新生血管,面积为每眼(0.06±0.12)mm2;生后17 d达到高峰,面积为每眼(1.79±0.02)mm2(χ2=20.112,P0.01)。Western blot检测结果显示,实验组VEGF表达不断增强,在生后17 d时表达达到高峰,为0.83±0.05。免疫组织化学染色结果显示,实验组生后12 d NF-κB阳性细胞计数为(12.32±1.04)个·mm-2,14 d时为(19.16±1.02)个·mm-2,生后17 d阳性表达较12 d和14 d时均高,为(28.60±0.52)个·mm-2(χ2=13.102,P0.05;χ2=20.132,P0.01)。结论 NF-κB在氧诱导血管增殖性视网膜病变小鼠视网膜中表达明显升高,其趋势与VEGF的变化相同,NF-κB参与了缺氧状态下视网膜新生血管形成的调控过程。
[Abstract]:Objective To investigate the expression of nuclear factor ( NF ) - 魏B in the retina of rats with proliferative retinopathy induced by oxygen . Methods 72 mice were randomly divided into experimental group ( 36 rats ) and control group ( 36 rats ) . The results of Western blot showed that the expression of VEGF in the experimental group was increased and the expression reached the peak at 17 days post - birth . The results showed that the positive expression of NF - 魏B in the experimental group was ( 12.32 卤 1.04 ) 路 mm - 2 , ( 19.16 卤 1.02 ) 路 mm - 2 at 14 days , and ( 28.60 卤 0.52 ) 路 mm - 2 ( 蠂 ~ 2 = 13.102 , P0.05 ; 蠂 ~ 2 = 20.132 , P0.01 ) . Conclusion The expression of NF - 魏B in the retina of proliferative diabetic retinopathy increased significantly , and its tendency was the same as that of VEGF . NF - 魏B was involved in the regulation of retinal neovascularization in hypoxia .
【作者单位】：南京中医药大学第三附属医院眼科;眼科国家重点实验室中山大学中山眼科中心;
【基金】：国家自然科学基金面上项目资助(编号:81270972) 南京市科技发展计划项目资助(编号:201308045) 南京市医学科技发展专项资金项目(医学科技发展重点项目)资助(编号:ZKX13047)~~
【分类号】：R774.1

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