炎症对声带间充质干细胞生物学行为的影响

发布时间：2018-06-02 09:48

本文选题：炎症 + 细胞外基质　；参考：《听力学及言语疾病杂志》2014年03期

【摘要】：目的研究炎症对声带间充质干细胞(vocal fold mesenchymal stem cells,VF-MSCs)生物学行为的影响,为炎症声带损伤修复提供一定的理论依据。方法正常和慢性炎症声带组织分别取自于下咽癌喉全切除的正常声带组织及水肿型声带息肉手术患者切除的标本,所取组织大小约1mm×1mm,每种组织3例,对其行HE、Masson三色染色和Elastin Van Gieson(EVG)染色,观察炎性声带组织较正常组织胶原纤维和弹性纤维改变情况;通过细胞克隆形成实验和MTT细胞生长曲线实验研究炎症对VF-MSCs增殖能力的影响;通过VF-MSCs定向向成骨和成脂细胞分化,研究炎症对VF-MSCs多向分化能力的影响。结果 Masson三色和EVG染色结果显示炎性声带组织固有层细胞外基质(extracellular matrix,ECM)胶原纤维变粗,含量增多,弹力纤维变细,含量减少;细胞克隆形成实验和MTT细胞生长曲线结果显示炎性声带间充质干细胞(inflammatory vocal fold mexenchymal stem cells,I-VF-MSCs)较正常声带间充质干细胞(normal vocal fold mesenchymal stem cells,N-VFMSCs)增殖能力增强;细胞成骨和成脂分化实验结果显示I-VF-MSCs较N-VF-MSCs多向分化能力减弱。结论炎性声带组织较正常声带组织弹性减弱,抗压力增强,炎症促进VF-MSCs增殖能力,抑制VF-MSCs多向分化能力。
[Abstract]:Objective to study the effects of inflammation on the biological behavior of vocal cord mesenchymal stem cells (fold mesenchymal stem) VF-MSCs, and to provide a theoretical basis for the repair of inflammatory vocal cord injury. Methods normal vocal cord tissue and chronic inflammatory vocal cord tissue were obtained from normal vocal cord tissue of hypopharyngeal carcinoma and patients with edematous vocal cord polyp resected respectively. The size of the tissue was about 1mm 脳 1 mm, 3 cases of each kind of tissue. The changes of collagen and elastic fibers in inflammatory vocal cord tissues were observed by means of Elastin Van Gieson staining and Elastin Van Gieson staining, and the effects of inflammation on the proliferation of VF-MSCs were studied by cell clone formation test and MTT cell growth curve test. The effects of inflammation on the multidirectional differentiation of VF-MSCs were studied by the differentiation of VF-MSCs into osteoblasts and adipoblasts. Results the results of Masson trichrome and EVG staining showed that the collagen fibers of the inflammatory vocal cord lamina propria extracellular matrix (ECM) became thicker, the content increased, the elastic fiber became thinner and the content decreased. The results of cell clone formation test and MTT cell growth curve showed that inflammatory vocal fold mexenchymal stem cells I-VF-MSCs (inflammatory vocal cord mesenchymal stem cells) enhanced the proliferative ability of normal vocal fold mesenchymal stem cells N-VFMSCs compared with normal vocal cord mesenchymal stem cells. The results of osteogenesis and adipogenic differentiation test showed that the multidirectional differentiation ability of I-VF-MSCs was weaker than that of N-VF-MSCs. Conclusion compared with normal vocal cord tissue, inflammatory vocal cord tissue has less elasticity and stronger anti-pressure. Inflammation promotes the proliferation of VF-MSCs and inhibits the multidirectional differentiation of VF-MSCs.
【作者单位】：第四军医大学西京医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金(81271065)资助
【分类号】：R767.4

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