高强度低频稳态噪声对大鼠耳蜗形态和功能影响的研究

发布时间：2018-06-05 10:27

本文选题：稳态噪声 + 低频　；参考：《中华耳科学杂志》2014年03期

【摘要】：目的探讨高强度低频稳态噪声对大鼠耳蜗形态和功能的影响。方法 42只SD大鼠随机分为2组,分别暴露于频率100Hz和300Hz,声压级153.7dB和158dB的声场中10分钟。暴露前、暴露后即刻、3天和7天分别测试大鼠听性脑干反应阈值(ABR),并取耳蜗标本行扫描电镜观察。结果 2组大鼠噪声暴露后听性脑干反应阈值均显著提高,脱离声场3天后仅可轻度恢复,7天后无进一步改善。300Hz组听力损伤更重。2组大鼠内外毛细胞均损伤严重,表现为毛细胞静纤毛缺失、融合及团状变,并可见螺旋器表面渗出性改变。由底圈向顶圈毛细胞损伤逐渐减轻,7天损伤程度较即刻有所减轻。300Hz组损伤较100Hz组重。结论高强度低频稳态噪声可造成大鼠耳蜗形态及功能严重损伤。耳蜗形态损伤以底圈最重,外毛细胞损伤较内毛细胞重。
[Abstract]:Objective to investigate the effect of high-intensity low-frequency steady-state noise on cochlea morphology and function in rats. Methods Forty-two Sprague-Dawley rats were randomly divided into two groups. They were exposed to the sound field of 100 Hz and 300 Hz at the sound pressure level of 153.7 dB and 158 dB for 10 minutes, respectively. The auditory brainstem response threshold was measured at 3 and 7 days before, immediately after exposure, and the cochlea specimens were taken for scanning electron microscopy. Results the threshold of auditory brainstem response was significantly increased after noise exposure in both groups. After 3 days of sound disconnection, there was no further improvement in the hearing loss in the group of .300Hz and the damage of hair cells inside and outside of the rats in the group of 3.00 Hz was more serious than that in the group of 3.00 Hz. The results were as follows: loss of static cilia, fusion and mass change of hair cells, and exudative change on the surface of spiral organ. The damage of hair cells from bottom circle to apical ring was gradually reduced after 7 days of injury. The damage in the group of .300Hz was more serious than that in the group of 100Hz. Conclusion High intensity low frequency steady-state noise can cause severe damage to cochlea morphology and function in rats. The damage of cochlea was the most serious in the bottom circle, and the damage of outer hair cells was more serious than that of inner hair cells.
【作者单位】：解放军306医院耳鼻咽喉头颈外科;解放军306医院消化内科;国防科技大学;
【分类号】：R764

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