外耳道及中耳胆脂瘤发生发展过程的动物实验研究

发布时间：2018-06-05 13:46

本文选题：中耳胆脂瘤 + 动物模型　；参考：《中国医科大学》2010年硕士论文

【摘要】： 目的应用蒙古长爪沙土鼠建立外耳道及中耳胆脂瘤模型并动态观察胆脂瘤的局部生长及破坏方式。为进一步研究胆脂瘤的发病机制并进行相关药物实验研究奠定基础。方法选取月龄2-6个月的雄性长爪蒙古沙土鼠50只,分为五组,每组10只动物双侧外耳道及鼓膜镜检均正常,全部于麻醉后行改良的右外耳道结扎术,左耳为对照耳。分别于术后15、30、45、60、90天解剖外耳道及双侧听泡,观察胆脂瘤在沙鼠外耳道及听泡内的生长及对周围结构的破坏情况并进行分期,再将胆脂瘤取出做组织病理学检查。结果 50只沙土鼠共成模38只,总成模率为76%,其中15天时成模率为50%,30天时成模率为60%,45天时成模率为80%,60天时成模率为90%,90天时成模率为100%；开放鼠双侧听泡,可见胆脂瘤生长进入右侧听泡内,并且随着时间延长侵犯范围逐渐扩大；组织病理检查见取出的标本有明显囊袋形成,囊袋外层由炎细胞和纤维组织包裹,内面为复层鳞状上皮,囊内含有大量脱落上皮及角化物,同时可见大量炎细胞浸润、粘液渗出及钙化灶,确定为胆脂瘤改变。结论应用外耳道结扎法可以很简便易行地建立外耳道及中耳胆脂瘤的动物模型,成模率较高；模型鼠胆脂瘤及其发生发展过程与人类胆脂瘤具有基本相同的特性；利用蒙古长爪沙鼠建立的外耳道及中耳胆脂瘤动物模型是研究胆脂瘤的发生发展及其破坏行为的理想试验材料,同时为进一步进行胆脂瘤相关药物实验研究奠定了良好的动物实验基础。
[Abstract]:Objective to establish the model of cholesteatoma in external auditory canal and middle ear of gerbils mongolicus and observe the local growth and destruction of cholesteatoma dynamically. Methods 50 male Mongolian gerbils aged 2-6 months were selected and divided into five groups. The bilateral external auditory meatus and tympanic membrane were all normal in each group of 10 animals, all of them were treated with modified right external auditory canal ligation after anesthesia, and the left ear was used as control ear. The external auditory meatus and bilateral auditory vesicles were dissected at 15: 30 and 45, 6090 days after operation. The growth of cholesteatoma in the external auditory canal and auditory vesicle of gerbils and the destruction of surrounding structures were observed and divided into stages. The cholesteatoma was removed for histopathological examination. The assembly mold rate is 76. In 15 days, the model forming rate is 50 and 30 days, and the model forming rate is 60 and 45 days. The model forming rate is 80 or 60 days and the model forming rate at 90 days is 100. When the bilateral auditory bubbles are opened, it can be seen that cholesteatoma grows into the right auditory vesicles. Histopathological examination showed that the specimens had obvious sac formation, the outer layer of the capsule was covered with inflammatory cells and fibrous tissue, and the inner surface was laminated squamous epithelium. The capsule contains a large number of exfoliative epithelium and keratocytes, as well as a large number of inflammatory cell infiltration, mucus exudation and calcification. Conclusion the animal model of external auditory canal and middle ear cholesteatoma can be easily established by ligation of external auditory canal and middle ear cholesteatoma. The model rat cholesteatoma and its occurrence and development process have the same characteristics as human cholesteatoma. The animal model of external auditory canal and middle ear cholesteatoma established by Mongolian gerbils is an ideal experimental material for studying the occurrence, development and destructive behavior of cholesteatoma. At the same time, it lays a good animal experimental foundation for the further study of cholesteatoma related drugs.
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R764.21

【参考文献】