miR-21、miR-98、miR-328和miR-494在喉鳞癌中的表达及其与肿瘤生物学特性的关系

发布时间：2018-06-08 12:15

本文选题：喉鳞癌 + miRNAs　；参考：《福建医科大学》2010年硕士论文

【摘要】： 【目的】观察miR-21、miR-98、miR-328和miR-494在喉鳞癌中的表达情况,探讨其与肿瘤生物学特性的关系及其临床意义,期望揭示喉鳞癌的发生、发展机理,为临床分子生物学治疗提供理论基础。【方法】用TaqMan real-time RT-PCR方法检测miRNAs在喉鳞癌及癌旁正常组织中的表达情况,并结合临床资料分析miRNAs与肿瘤生物学特性的关系。【结果】1.miR-21, miR-98, miR-328和miR-494在喉鳞癌及癌旁正常组织均有表达,表达量各不相同,miR-21的表达量明显高于其他三个的表达量。2.miR-21在喉鳞癌中的表达量明显高于癌旁正常组织(P0.01);而miR-98、miR-328和miR-494在喉鳞癌中的表达与在癌旁正常组织中表达无明显差异性(P0.05)。3.miR-21的表达量与肿瘤TNM分期有关(HC=9.272,P0.05);miR-21在有颈部淋巴结转移的肿瘤组织中的表达量明显高于没有颈部淋巴结转移的肿瘤组织(HC=14.863,P0.05);miR-21在病理分级中表达有差异性(HC=9.871,P0.05);未发现miR-21在肿瘤中的表达与吸烟(U = 1.271,P0.05)、临床分型(U = 1.328,P0.05)有关。【结论】miR-21在肿瘤组织中表达越高,淋巴结转移的可能性越大,且与肿瘤TNM分期及病理分级有关,与吸烟、临床分型等因素无关。miR-21与喉鳞癌的发生、发展有密切关系,并可能与喉鳞癌的预后有关。miR-98、miR-328和miR-494可能与喉鳞癌的形成无关。
[Abstract]:[objective] to observe the expression of miR-21 miR-98 miR-328 and miR-494 in laryngeal squamous cell carcinoma (LSCC), and to explore the relationship between miR-21 miR-98miR-328 and miR-494 in laryngeal squamous cell carcinoma (LSCC) and its clinical significance, in order to reveal the pathogenesis of laryngeal squamous cell carcinoma (LSCC). [methods] TaqMan real-time RT-PCR was used to detect the expression of miRNAs in laryngeal squamous cell carcinoma and adjacent normal tissues. [results] 1. MiR-21, miR-98, miR-328 and miR-494 were expressed in laryngeal squamous cell carcinoma and adjacent normal tissues. The expression of miR-21 in laryngeal squamous cell carcinoma was significantly higher than that of the other three. 2. The expression of miR-21 in laryngeal squamous cell carcinoma was significantly higher than that in adjacent normal tissue (P0.01), while the expression of miR-98 miR-328 and miR-494 in laryngeal squamous cell carcinoma was not clear. The expression of HC9. 272 and P0. 05 miR-21 in tumor tissues with cervical lymph node metastasis was significantly higher than that in tumor tissues without cervical lymph node metastasis. There was no difference in the expression of HC9. 871 and P0. 05 in pathological grade. It was found that the expression of miR-21 in the tumor was related to the expression of miR-21 in the tumor tissues. [conclusion] the higher the expression of miR-21 in the tumor tissues, the higher the expression of miR-21 in the tumor tissues. The higher the possibility of lymph node metastasis, and the more related to TNM stage and pathological grade of tumor, the more closely related to the occurrence and development of laryngeal squamous cell carcinoma, but not related to smoking, clinical type and other factors. It may be related to the prognosis of laryngeal squamous cell carcinoma. MiR-98 miR-328 and miR-494 may not be related to the formation of laryngeal squamous cell carcinoma.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65

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