慢性扁桃体炎中人β-防御素-3的表达及意义

发布时间：2018-06-09 15:37

本文选题：慢性扁桃体炎 + 人β-防御素-　；参考：《兰州大学学报(医学版)》2013年02期

【摘要】：目的探讨慢性扁桃体炎中人β-防御素-3(hBD-3)的表达及意义。方法应用HE染色,逆转录聚合酶链式反应、SP法、蛋白印迹法观察和检测15例扁桃体炎组和10例对照组组织的病理变化及组织中hBD-3的表达。结果 HE光镜下显示对照组病理特点固有层纤维结缔组织反应性增生为主,非角化复层扁平层内未见炎性细胞浸润;扁桃体炎组淋巴滤泡增生,生发中心扩大,非角化复层扁平层内炎性细胞浸润,蛋白渗出明显。逆转录聚合酶链式反应结果显示扁桃体炎组观察到目的片段;对照组未见hBD-3的特异片段。免疫组织化学光镜下显示扁桃体炎组非角化复层扁平上皮细胞胞浆中hBD-3阳性表达;对照组非角化复层扁平上皮细胞胞浆中呈阴性表达。蛋白印迹法检测结果显示扁桃体炎组可见阳性条带;对照组未见阳性条带。结论 hBD-3在慢性扁桃炎中的表达,表明hBD-3可能在扁桃体和上呼吸道抗感染中发挥重要作用。
[Abstract]:Objective to investigate the expression and significance of human 尾-defensin-3 hBD-3 in chronic tonsillitis. Methods the histopathological changes and the expression of hBD-3 in 15 cases of tonsillitis and 10 cases of control group were observed and detected by HE staining, reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting. Results under HE light microscope, the pathological features of the control group were fibrous connective tissue reactive hyperplasia in lamina propria, no inflammatory cell infiltration in the flat layer of non-keratinized lamina, lymphoid follicular hyperplasia and enlargement of germinal center in tonsillitis group. Inflammatory cells infiltrated in the flat layer of non-keratinized lamina, and protein exudation was obvious. Reverse transcriptase polymerase chain reaction showed that the target fragment was observed in tonsillitis group, but no specific hBD-3 fragment was found in control group. The expression of hBD-3 in cytoplasm of non-keratinized lamellar flat epithelial cells in tonsillitis group and control group was negative. The positive bands were found in tonsillitis group and control group. Conclusion the expression of hBD-3 in chronic tonsillitis suggests that hBD-3 may play an important role in anti-infection of tonsil and upper respiratory tract.
【作者单位】：兰州大学基础医学院甘肃省新药临床前研究重点实验室;
【分类号】：R766.18

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