NDRG1在鼻咽癌组织中的表达及其意义

发布时间：2018-06-10 05:57

本文选题：鼻咽肿瘤 + NDRG1　；参考：《昆明医学院》2011年硕士论文

【摘要】：目的探讨人N-myc下游调节基因1 (N-myc down-regulated gene 1, NDRG1)在鼻咽癌组织中的表达情况,以及与临床病理指标和预后的关系。方法应用免疫组化SP法检测80例鼻咽癌和20例鼻咽慢性炎性组织中NDRG1蛋白的表达情况；应用实时荧光定量PCR (real-time PCR)检测10例鼻咽癌和10例鼻咽慢性炎性组织中NDRG1 mRNA的表达情况；分析NDRG1基因与鼻咽癌临床病理特征及预后的关系。结果免疫组化结果显示,在鼻咽癌组织中,NDRG1蛋白表达阳性率为88.8%(71/80),在鼻咽慢性炎性组织中阳性率为100%(20/20),并且在鼻咽癌组织中NDRG1蛋白多为低表达,73.8%(59/80),而在鼻咽慢性炎性组织低表达率仅为10%(2/20)；荧光定量PCR结果显示,2-△△Ct=0.25(0.14～0.45),即表示NDRG1 mRNA在鼻咽慢性炎性组织中的相对表达量约为鼻咽癌组织的4倍。NDRG1蛋白表达与鼻咽癌区域淋巴结转移、TNM分期具有相关性,而与患者年龄、性别、浸润程度、远处转移无关。NDRG1蛋白低表达鼻咽癌患者预后较高表达者差,5年总生存率和无进展生存率分别为42.3%和26.9%。结论NDRG1基因在鼻咽癌组织中多呈低表达,与肿瘤的转移、临床分期密切相关,可作为鼻咽癌转移的一个候选抑癌基因；可作为鼻咽癌预后评价的分子标志物之一；并且可能作为一个潜在的治疗靶点。
[Abstract]:Objective to investigate the expression of N-myc down-regulated gene 1 (NDRG1) in nasopharyngeal carcinoma (NPC). Methods Immunohistochemical SP method was used to detect the expression of NDRG1 protein in 80 nasopharyngeal carcinoma and 20 nasopharyngeal chronic inflammatory tissues. The expression of NDRG1 mRNA in 10 cases of nasopharyngeal carcinoma and 10 cases of nasopharyngeal chronic inflammatory tissue was detected by real-time PCR, and the relationship between NDRG1 gene and clinicopathological features and prognosis of nasopharyngeal carcinoma was analyzed. The positive rate of NDRG1 protein expression in nasopharyngeal carcinoma tissue was 88.8 / 71 / 80, in nasopharyngeal chronic inflammatory tissue was 100 / 20 / 20, and in nasopharyngeal carcinoma tissue, NDRG1 protein expression was low 73.8% / 80%, while in nasopharyngeal chronic inflammatory tissue, the low expression rate was 1022 / 20%. The quantitative PCR results showed that the relative expression of NDRG1 mRNA in nasopharyngeal chronic inflammatory tissues was about 4 times higher than that in nasopharyngeal carcinoma tissues. The expression of NDRG1 protein was correlated with the TNM stage of regional lymph node metastasis of nasopharyngeal carcinoma. The prognosis of NPC patients with low expression of NDRG1 protein and age, sex, infiltration degree, distant metastasis was lower than that of patients with NPC. The 5-year overall survival rate and progression-free survival rate were 42.3% and 26.9G respectively. Conclusion NDRG1 gene is mostly low expression in nasopharyngeal carcinoma tissues. It can be used as a candidate tumor suppressor gene for metastasis of nasopharyngeal carcinoma, as a molecular marker for prognosis evaluation of nasopharyngeal carcinoma, and as a potential therapeutic target.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.63

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