佛手柑内酯对鼻咽癌细胞凋亡的影响

发布时间：2018-06-10 10:46

本文选题：佛手柑内酯 + 鼻咽癌　；参考：《肿瘤防治研究》2014年11期

【摘要】：目的探讨佛手柑内酯对鼻咽癌细胞凋亡的影响及可能的作用机制。方法 CCK-8法检测佛手柑内酯对鼻咽癌细胞CNE-2和HONE-1体外活性的抑制作用。Annexin V-FITC/PI双染法、JC-1染料法分别检测细胞的凋亡率及线粒体膜电位,并检测Caspase-3的活性水平。实时定量RT-PCR与Western blot检测BAX与BCL2基因的mRNA水平和蛋白表达量,并检测STAT3蛋白表达量及其Y705位点的磷酸化水平。结果在10μM和100μM两个浓度佛手柑内酯作用下,CNE-2和HONE-1细胞凋亡率及Caspase-3活性明显上升(P0.05),线粒体膜电位明显下降(P0.05);BCL2基因的mRNA及蛋白表达水平降低,BAX基因的mRNA及蛋白表达水平升高,STAT3蛋白表达量不变而其Y705位点的磷酸化水平下降。结论佛手柑内酯能破坏鼻咽癌细胞线粒体功能并诱导其发生凋亡,这一过程可能与下调BCL2基因、上调BAX基因及抑制STAT3磷酸化有关。
[Abstract]:Objective to investigate the effect of bergamot lactone on apoptosis of nasopharyngeal carcinoma cells and its possible mechanism. Methods the inhibitory effect of bergamot lactone on CNE-2 and HONE-1 cells was detected by CCK-8 method. Annexin V-FITC / Pi double staining method was used to detect the apoptosis rate and mitochondrial membrane potential, and the activity level of Caspase-3 was detected. The mRNA and protein expression of Bax and BCL2 genes were detected by real-time quantitative RT-PCR and Western blot. The expression of STAT3 protein and phosphorylation of Y705 locus were also detected. Results the apoptotic rate and Caspase-3 activity of CNE-2 and HONE-1 cells were significantly increased under the action of 10 渭 M and 100 渭 M bergamot lactone, while the mitochondrial membrane potential decreased the mRNA and protein expression level of BCL2 gene of P0.05, and decreased the mRNA and protein expression of BCL2 gene. The level of phosphorylation at Y705 site was decreased. Conclusion bergamot lactone can damage mitochondrial function and induce apoptosis in nasopharyngeal carcinoma cells, which may be related to down-regulation of BCL2 gene, up-regulation of Bax gene and inhibition of STAT3 phosphorylation.
【作者单位】：广东医学院生物化学与分子生物学教研室;
【基金】：国家自然科学基金(81272434) 湛江市科技攻关计划(2013B01091) 广东医学院青年基金(Q2012005)
【分类号】：R739.63

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