扁桃体淋巴细胞归巢异常与IgAN发病机制的研究

发布时间：2018-06-10 12:43

本文选题：IgA肾病 + 扁桃体　；参考：《华中科技大学》2011年硕士论文

【摘要】：目的:探讨L-选择素,VLA-4α,LFA-1,ICAM-1,VCAM-1在IgA肾病(IgA nephropathy IgAN)患者与非肾炎慢性扁桃体炎患者扁桃体的表达及意义。方法:收集10例伴有慢性扁桃体炎的IgAN患者扁桃体(IgAN组),这些患者经肾活检诊断为IgAN,10例不伴IgAN慢性扁桃体炎(Chronic tonsillitis CT组)作为对照。应用Western Blot技术定量分析两组扁桃体组织L-选择素,VLA-4α,LFA-1,ICAM-1,VCAM-1的表达,同时采用免疫组织化学技术检测扁桃体L-选择素,VLA-4α,LFA-1表达,观察ICAM-1,VCAM-1在生发中心和滤泡间区高内皮小静脉(High endothelial venules HEV)分布。采用SPSS13.0软件进行统计学分析。结果:1.Western blot技术显示IgAN组及CT组扁桃体L-选择素,VLA-4α,LFA-1, ICAM-1,VCAM-1的表达分别为〔(1.10±0.09;0.80±0.14),(0.39±0.05;0.24±0.04), ( 0.41±0.06;0.29±0.06),(1.36±0..04;1.18±0.09), ( 2.56±0.63;1.60±0.32)〕。2.免疫组织化学检测两组各归巢受体表达差别有显著统计学意义,IgAN扁桃体生发中心和滤泡外区HEV内血管地址素表达增强。结论:扁桃体淋巴细胞归巢异常是伴有慢性扁桃体炎的IgAN患者的重要特征,IgAN的发病机制与之有密切关联。
[Abstract]:Objective: to investigate the expression and significance of L- selectin VLA-4 伪 in the tonsils of patients with IgA nephropathy and chronic tonsillitis. Methods: ten patients with chronic tonsillitis and 10 patients with chronic tonsillitis were collected. Ten patients with chronic tonsillitis without IgAN were diagnosed by renal biopsy as control group. The expression of L- selectin (VLA-4 伪) and ICAM-1 (VCAM-1) in tonsils were quantitatively analyzed by Western blot, and the expression of L- selectin VLA-4 伪 -lFA-1 in tonsils was detected by immunohistochemical technique. The distribution of ICAM-1 VCAM-1 in high endothelial venules was observed in the germinal center and interfollicular area. Results 1.Western blot technique showed that the expression of L- selectin in tonsils of IgAN group and CT group was 1.10 卤0.09 卤0.80 卤0.140.24 卤0.04 卤0.04 卤0.04 卤1.18 卤0.09, (2.56 卤0.631.60 卤0.32), (0.41 卤0.06 卤0.29 卤0.06) 卤1.18 卤0.09, (2.56 卤0.631.60 卤0.32), respectively. 2. The expression of VCAM-1 in the tonsils of IgAN group and CT group was 1.18 卤0.09, (2.56 卤0.631.60 卤0.32), 0.39 卤0.050.24 卤0.04, (0.41 卤0.06, 0.29 卤0.06, 1.18 卤0.09, 2.56 卤0.63, 1.60 卤0.32, respectively). The difference of homing receptor expression between the two groups was statistically significant. Conclusion: the homing abnormality of tonsil lymphocytes is accompanied by slow homing in IgAN tonsil germinal center and extrafollicular area of HEV. An important feature of IgAN patients with tonsillitis is the pathogenesis of IgAN.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R692.3;R766.18

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