荧光定量RT-PCR检测新疆维、汉人群变应性鼻炎中IL-2及IL-4mRNA的表达水平

发布时间：2018-06-11 11:09

本文选题：变应性鼻炎 + IL-　；参考：《免疫学杂志》2014年05期

【摘要】：目的检测新疆维、汉人群变应性鼻炎(AR)患者外周血CD4+T淋巴细胞中IL-2及IL-4 mRNA的表达水平,比较2种基因在该疾病中的作用。方法应用实时荧光定量RT-PCR技术检测80例变应性鼻炎患者和80例正常对照者IL-2及IL-4mRNA的表达水平,其中鼻炎患者和对照者中维族、汉族各40例。结果选择管家基因GAPDH进行校正,IL-2 mRNA表达水平在AR组为(8.66±1.92)coples/μl,维族和汉族分别为(8.52±2.15)和(8.81±1.79)coples/μl,AR对照组为(11.28±1.78)coples/μl,其中维族和汉族分别为(10.31±1.92)和(12.27±0.93)coples/μl。IL-4 mRNA的表达水平经管家基因GAPDH校正后在AR组为(10.90±2.54)coples/μl,其中维族和汉族分别为(10.42±2.95)和(11.41±2.13)coples/μl,AR对照组为(3.07±1.18)coples/μl,维族和汉族分别为(2.81±1.46)和(3.46±0.61)coples/μl。AR组IL-2 mRNA的基因表达水平与健康对照者之间有差异(P0.05),维吾尔族和汉族之间无明显差异(P0.05)。AR组IL-4 mRNA的基因表达水平高于健康对照者(P0.05),在维族汉族之间差异不大(P0.05)。结论 IL-2 mRNA基因表达水平在AR组和健康对照者之间有显著差异(P0.05),IL-4 mRNA的基因表达水平高于健康对照者。IL-2和IL-4基因在变应性鼻炎的发病过程中作用显著,IL-2和IL-4基因在维吾尔族和汉族AR患者之间无明显差异(P0.05)。
[Abstract]:Objective to detect the expression of IL-2 and IL-4 mRNA in peripheral blood CD4 T lymphocytes of patients with allergic rhinitis in Xinjiang and to compare the roles of two genes in the disease. Methods the expression of IL-2 and IL-4 mRNA in 80 patients with allergic rhinitis and 80 normal controls were detected by real-time fluorescence quantitative RT-PCR, including 40 cases of Uygur nationality and 40 cases of Han nationality. Results the levels of IL-2 mRNA expression in AR group were 8.66 卤1.92)coples/ 渭 l, 8.52 卤2.15 in Uygur group and 8.81 卤1.79)coples/ 渭 1.78)coples/ 渭 l in Han nationality, respectively. The expression levels of IL-4 mRNA in Uygur group and Han nationality were 10.31 卤1.92) and 12.27 卤0.93)coples/ 渭 l. IL-4 mRNA levels were corrected by GAPDH. The mRNA expression level of IL-2 in Uygur group and Han nationality group was 10.90 卤2.54)coples/ 渭 l, respectively, 10.42 卤2.95) and 11.41 卤2.13)coples/ 渭 l 渭 l in Uygur group, 2.81 卤1.46 渭 l in Uygur group and 3.46 卤0.61)coples/ 渭 l.AR group, respectively. There was no significant difference in IL-2 gene expression between Uygur group and Han nationality group. There was no significant difference between Uygur group and Han nationality group. The gene expression level of IL-4 mRNA was higher than that of healthy controls (P 0.05), but there was no significant difference between Uygur and Han nationality. Conclusion the level of IL-2 mRNA gene expression in AR group is significantly higher than that in healthy control group. IL-2 and IL-4 genes play a significant role in the pathogenesis of allergic rhinitis. There was no significant difference in AR between Uygur and Han nationality (P 0.05).
【作者单位】：新疆医科大学中医学院;新疆医科大学第六附属医院呼吸科;
【基金】：国家自然科学基金项(81260006)
【分类号】：R765.21

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