豚鼠声刺激诱发的前庭咬肌反射

发布时间：2018-06-12 15:47

本文选题：前庭 + 声刺激　；参考：《华中科技大学》2010年博士论文

【摘要】： 第一部分声刺激诱发麻醉豚鼠前庭咬肌反射及起源的研究目的：建立一个声诱发前庭咬肌反射的动物模型,并通过手术切断前庭下神经及破坏耳蜗神经来确定该电位的起源。方法：20只豚鼠,随机分为3组,正常对照组10只,单侧前庭下神经切断组5只,单侧耳蜗神经破坏组5只。3组动物在麻醉下,记录Click声诱发的咬肌反射电位及听性脑干反应测试(ABR)。在记录咬肌反射电位的过程中,在豚鼠下颌骨及颅顶之间用金属夹夹住,使咬肌保持一定的张力。结果：正常对照组声诱发咬肌反射电位的负波(negative peak, NP)阈值为92±7.68dBnHL给予100、90、80、70dBnHL单侧声刺激时,同侧记录咬肌反射NP引出率分别为100%、70%、40%、0。给予100、90、80dBnHL单侧声刺激时,同侧记录咬肌反射NP平均潜伏期分别为6.57±0.26ms,6.64±0.23ms,6.69±0.19ms。不同刺激强度,咬肌反射NP潜伏期差异无统计学意义(P0.05)。未给肌紧张时,该反射消失,给予肌紧张时,该反射存在。ABR的平均阈值为31±7.88 dBnHL。单侧前庭下神经切断组,术侧声诱发咬肌反射消失,ABR反应阈在正常范围内。单侧耳蜗神经破坏组,术侧声诱发咬肌反射存在,与正常对照组相比,NP阈值及潜伏期差异无统计学意义(P0.05),该组ABR消失。结论：声刺激诱发的豚鼠咬肌反射电位NP来源于前庭,而且该反射是肌源性的,通过Click声以及在豚鼠下颌骨及颅顶之间应用金属夹,使咬肌保持一定的张力的方法,可以建立一个理想的声刺激诱发前庭咬肌反射的动物模型。第二部分豚鼠三叉神经下颌支部分传入神经切断对声诱发咬肌反射的影响目的：研究麻醉豚鼠三叉神经下颌支部分传入神经切断对声诱发的咬肌反射的影响。方法：15只豚鼠随机分成3组,正常对照组5只,单侧下牙槽神经切断5只,单侧耳颞神经切断5只。3组动物分别进行Click声刺激诱发的前庭咬肌反射检测。结果：正常对照组咬肌反射的阈值为90±8.16dBnHL。100、90、80、70 dBnHL单侧声刺激,同侧记录咬肌反射的引出率分别为100%、70%、40%、0。100、90、80dBnHL单侧声刺激咬肌反射的潜伏期分别为6.55±0.25ms,6.61±0.16ms,6.7±0.13ms。不同刺激强度咬肌反射的潜伏期差异无统计学意义(P0.05)。单侧下牙槽神经及单侧耳颞神经切断,术侧咬肌反射的阈值,与正常对照组相比,潜伏期差异无统计学意义(P0.05)。结论：声诱发的咬肌反射不受三叉神经下颌支耳颞神经及下牙槽神经传入纤维的影响。
[Abstract]:Part I study on the origin and origin of vestibular masseter reflex in anesthetized guinea pigs objective: to establish an animal model of vestibular masseter reflex induced by sound stimulation. The origin of the potential was determined by cutting off the inferior vestibular nerve and destroying the cochlear nerve. Methods Twenty guinea pigs were randomly divided into 3 groups: normal control group (n = 10), unilateral subvestibular nerve transection group (n = 5) and unilateral cochlear nerve destruction group (n = 5) under anesthesia. The response of masseter muscle induced by Click and auditory brainstem response were recorded. In the course of recording the reflex potential of masseter muscle, a metal clamp was used between the mandible and cranium of the guinea pig to maintain the tension of the masseter muscle. Results: the negative peak (NPK) threshold of acoustic evoked masseter reflex potential in normal control group was 92 卤7.68 dBnHL. When unilateral acoustic stimulation was performed, the NP extraction rate of ipsilateral recorded masseter reflex was 100 卤7.68 dBnHL and 100 卤7.68 dBnHL, respectively. The average latency of NP in ipsilateral recorded masseter reflex was 6.57 卤0.26ms 6.64 卤0.23ms / 6.69 卤0.19msrespectively. There was no significant difference in NP latency of masseter reflex with different stimulation intensity (P 0.05). The mean threshold of the reflex was 31 卤7.88 dBnHL when the tension was not given and the mean threshold of ABR was 31 卤7.88 dBnHL. In unilateral subvestibular nerve transection group, the ABR reaction threshold was within normal range. In the unilateral cochlear nerve destruction group, the acoustic evoked masseter reflex was found in the operative side. There was no significant difference in NP threshold and latency between the two groups (P 0.05), and ABR disappeared in this group. Conclusion: the reflex potential NP of guinea pig masseter induced by sound stimulation comes from vestibule, and the reflex is myogenic. By using click sound and metal clip between the mandible and cranium of guinea pig, the masseter muscle can maintain a certain tension. An ideal animal model of vestibular masseter reflex induced by sound stimulation can be established. Part two effects of partial afferent nerve transection of mandibular branch of trigeminal nerve on acoustic evoked masseter reflex in guinea pig objective: to study the effects of partial afferent nerve transection of mandibular branch of trigeminal nerve on acoustic evoked reflex in anesthetized guinea pig trigeminal nerve The effect of masseter reflex. Methods Fifteen guinea pigs were randomly divided into 3 groups: normal control group (n = 5), unilateral inferior alveolar nerve transection (n = 5) and temporal nerve transection (n = 5). Results: the threshold of masseter reflex in normal control group was 90 卤8.16dBnHL.100,80,80,70dBnHL unilateral acoustic stimulation, and the extraction rate of ipsilateral recorded masseter reflex was 1000.100dBnHL 0.100,90,90dBnHL, the latency of masseter reflex was 6.55 卤0.25ms 6.61 卤0.16msL 6.7 卤0.13msrespectively. There was no significant difference in latency of masseter reflex with different stimulation intensity (P 0.05). There was no significant difference in latency between unilateral inferior alveolar nerve and unilateral auricular temporal nerve and the threshold of masseter reflex in surgical side compared with the control group (P 0.05). Conclusion: the acoustic evoked masseter reflex is not affected by the afferent fibers of the mandibular branch temporal nerve and inferior alveolar nerve of the trigeminal nerve.
【学位授予单位】：华中科技大学
【学位级别】：博士
【学位授予年份】：2010
【分类号】：R764

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