水杨酸钠对大鼠海马神经元电压门控性钙离子通道的影响

发布时间：2018-06-12 23:28

本文选题：水杨酸钠 + 耳鸣　；参考：《中国药理学通报》2014年09期

【摘要】：目的研究水杨酸钠对大鼠海马神经元上电压门控性钙离子通道的作用,为海马参与耳鸣引起的情绪变化提供电生理学的依据。方法利用脑片膜片钳技术观察水杨酸钠对大鼠海马神经元电压门控性钙离子通道电生理学特性的影响。结果水杨酸钠对电压门控性钙离子通道电流(ICa)有抑制作用,而且具有浓度依赖性(0.1~10 mmol·L-1)。水杨酸钠对海马神经元ICa的半抑制浓度(IC50)为1.64。1mmol·L-1水杨酸钠对ICa的稳态激活动力学有明显影响,使ICa的稳态激活曲线向超极化方向移动大约9 mV左右,但是对其稳态失活动力学没有明显影响。结论水杨酸钠以浓度依赖的方式抑制ICa,对其稳态激活动力学有明显影响。水杨酸钠对大鼠海马神经元上ICa的影响可能与海马参与耳鸣引起的情绪变化相关。
[Abstract]:Aim to study the effect of sodium salicylate on voltage-gated calcium channels in rat hippocampal neurons and to provide electrophysiological evidence for the participation of hippocampus in the emotional changes induced by tinnitus. Methods the effects of sodium salicylate on the electrophysiological characteristics of voltage-gated calcium channels in rat hippocampal neurons were observed by using patch clamp technique. Results Sodium salicylate inhibited the current of voltage-gated calcium channel (ICA) in a concentration-dependent manner with a concentration dependence of 0.1 ~ (10) mmol / L ~ (-1). The semi-inhibitory concentration of sodium salicylate on IC _ (50) in hippocampal neurons is that sodium salicylate 1.64.1mmol L ~ (-1) has a significant effect on the steady-state activation kinetics of IC _ (1), which makes the steady-state activation curve of IC _ (a) move about 9 MV in hyperpolarization direction. However, there is no obvious effect on the steady-state inactivation kinetics. Conclusion Sodium salicylate inhibits ICA in a concentration-dependent manner and has a significant effect on its steady-state activation kinetics. The effect of sodium salicylate on IC _ a in rat hippocampal neurons may be related to the emotional changes induced by tinnitus.
【作者单位】：河北医科大学药理学教研室河北省新药药理毒理研究重点实验室河北省哈励逊国际和平医院
【基金】：国家自然科学基金资助项目(No 30600125) 河北省自然科学基金资助项目(No C2011206036)
【分类号】：R764.45

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