多西环素对碱烧伤大鼠角膜炎症细胞浸润的抑制作用

发布时间：2018-06-13 23:29

本文选题：多西环素 + 碱烧伤　；参考：《国际眼科杂志》2013年06期

【摘要】：目的:观察多西环素对碱烧伤大鼠角膜炎症细胞浸润的抑制作用。方法:健康SD大鼠32只,随机分为对照组、多西环素组,每组16只大鼠。建立角膜碱烧伤模型后,多西环素组予3g/L多西环素眼液点眼,对照组予眼液溶媒点眼。分别于碱烧伤后第3,7,14,21d观察计算炎症指数,角膜取材后进行病理切片及炎症细胞计数,行ICAM-1的ELISA检测。结果:多西环素组各时间点结膜充血、角膜水肿较对照组轻,炎症指数均明显低于对照组,差异有统计学意义(P0.05)。多西环素组各时间点炎症细胞计数少于对照组(P0.05)。多西环素组各时间点大鼠角膜的ICAM-1表达低于对照组(P0.05)。结论:多西环素可以抑制碱烧伤大鼠角膜的炎症细胞浸润,其机制可能是抑制ICAM-1的表达。
[Abstract]:Aim: to observe the inhibitory effect of doxycycline on corneal inflammatory cell infiltration in rats with alkali burn. Methods: Thirty-two healthy SD rats were randomly divided into control group (n = 16) and doxycycline group (n = 16). After the corneal alkali burn model was established, the doxycycline group was given 3 g / L doxycycline eye drops eye, and the control group was given eye solution point eye. The inflammatory index was observed and calculated on the 3rd day, 14th day, 21st day after alkali burn. The pathological section and inflammatory cell count were taken from the cornea, and the ICAM-1 was detected by Elisa. Results: the conjunctival hyperemia and corneal edema in doxycycline group were lighter than those in control group, and the inflammatory index was significantly lower than that in control group (P 0.05). The number of inflammatory cells in the doxycycline group was less than that in the control group at each time point (P 0.05). The expression of ICAM-1 in cornea of doxycycline group was lower than that of control group (P 0.05). Conclusion: doxycycline can inhibit the infiltration of inflammatory cells in the cornea of rats with alkali burn, and its mechanism may be to inhibit the expression of ICAM-1.
【作者单位】：广西医科大学第一附属医院眼科;
【基金】：国家自然科学基金(No.81160119) 广西自然科学基金(No.2012GXNSFAA053107) 广西高等学校立项科研项目(No.201204LX064)~~
【分类号】：R772.21

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