乙酰半胱氨酸对角膜新生血管形成过程中瘦素和PEDF表达的调节

发布时间：2018-06-14 14:14

本文选题：碱烧伤 + 角膜新生血管　；参考：《眼科新进展》2014年08期

【摘要】：目的观察大鼠碱烧伤后不同时期角膜组织病理学变化、瘦素和色素上皮衍生因子(pigment epithelium derived factor,PEDF)的表达以及乙酰半胱氨酸对瘦素和PEDF表达的影响,探讨他们与角膜新生血管(corneal neovascularization,CNV)的关系。方法使用碱烧伤诱导大鼠CNV模型,采用浸润1 mol·L-1NaOH溶液,直径为3.0 mm的单片滤纸,准确置于大鼠右眼角膜中央23 s制作CNV模型。75只大鼠随机分成3组,每组25只,左眼为正常对照眼。A组自烧伤之日起每天滴注PBS液;B组每天滴注乙酰半胱氨酸;C组烧伤后7 d之内每天滴注PBS液,7 d后每天加滴乙酰半胱氨酸。用裂隙灯显微镜观察CNV,分别计算CNV长度和面积,并行HE染色和免疫组织化学检测。结果 B组CNV生长趋势与A组、C组基本相似,但较A组、C组生长迟缓,C组比A组缓慢,但稍快于B组。C组角膜病理变化与A组相似,较B组稍重。除4 d时A组和C组间CNV长度及面积差异均无统计学意义(均为P0.05)外,余时间点组间两两比较差异均有统计学意义(均为P0.05)。免疫组织化学检测显示角膜碱烧伤后,瘦素和PEDF的表达开始增高。伤后7 d,各组瘦素表达:A组、B组、C组分别为0.276 1±0.114 4、0.232 2±0.074 9、0.269 2±0.064 6,PEDF表达分别为0.352 1±0.107 4、0.389 9±0.072 1、0.356 1±0.123 3,三组瘦素、PEDF表达总体比较差异有统计学意义(P0.05);两两比较显示,A组与B组、B组与C组瘦素、PEDF表达差异均有统计学意义(均为P0.05)。伤后14 d,各组瘦素与PEDF的表达总体比较差异均有统计学意义(均为P0.05);各组间两两比较显示,瘦素、PEDF表达差异均有统计学意义(均为P0.05)。结论瘦素和PEDF表达水平与CNV的生成具有相关性,乙酰半胱氨酸能有效降低瘦素的表达,提高PEDF的表达。
[Abstract]:Objective to observe the histopathological changes of cornea, the expression of leptin and pigment epithelium-derived factor pigment epithelium derived factor (PEDF) and the effect of acetylcysteine on the expression of leptin and PEDF in rats after alkali burn. To investigate their relationship with corneal neovascularization (CNV). Methods the rat model of CNV was induced by alkali burn. The rats were randomly divided into 3 groups with 25 rats in each group, which were treated with 1 mol L -1 NaOH solution and 3. 0 mm diameter filter paper, and placed accurately in the central cornea of right eye for 23 s. The left eye was the normal control group. Group A was treated with PBS solution daily from the day of burn. Group B was infused with acetylcysteine daily within 7 days after burn, and then added acetylcysteine daily after 7 days of burn. The length and area of CNV were calculated by slit lamp microscope. Results the growth trend of CNV in group B was similar to that in group A and C, but slower than that in group A and C, but a little faster than that in group B and group C. the pathological changes of cornea in group B were similar to those in group A and were a little heavier than those in group B. There was no significant difference in CNV length and area between group A and group C at 4 days (P0.05), but there were significant differences between two groups at other time points (P0.05). Immunohistochemical examination showed that the expression of leptin and PEDF began to increase after alkali burn of cornea. On the 7th day after injury, the expression of leptin in group B and C was 0.276 1 卤0.114 4, 0.232 2 卤0.074 9, 0.269 2 卤0.064 6, respectively, 0.352 1 卤0.107 40.389 9 卤0.072 1 卤0.356 1 卤0.123 3. There was a significant difference in the expression of leptin in three groups (P 0.05), and two comparisons showed that the expression of leptin in group A and group B was significantly higher than that in group B (P < 0.05), and the expression of leptin in group B was significantly higher than that in group B (P < 0.05), while the expression of leptin in group B was significantly different from that in group A (0.352 1 卤0.107). The difference of PEDF expression between group C and group C was statistically significant (P 0.05). On the 14th day after injury, there were significant differences in the expression of leptin and PEDF in all groups (all P 0.05), and the differences in the expression of leptin and PEDF among the groups were all statistically significant (P 0.05). Conclusion the expression levels of leptin and PEDF are correlated with CNV formation. Acetylcysteine can effectively decrease the expression of leptin and increase the expression of PEDF.
【作者单位】：石河子大学医学院;石河子大学附属医院眼科;
【分类号】：R779.13

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