糖尿病合并高度近视患者玻璃体中VEGF和EPO的表达及意义

发布时间：2018-06-17 16:42

本文选题：糖尿病视网膜病变 + 高度近视　；参考：《眼科新进展》2014年05期

【摘要】：目的通过检测高度近视合并糖尿病患者玻璃体中血管内皮生长因子(vascular endotheial growth factor,VEGF)和促红细胞生成素(erythropoietin,EPO)的浓度,探讨高度近视对糖尿病视网膜病变的影响。方法选取待行玻璃体手术的视网膜脱离患者62例(62眼),其中对照组18眼,高度近视组16眼,增生型糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)组15眼,高度近视合并糖尿病组13眼,对玻璃体切割术中取出的玻璃体原液采用双抗体夹心酶联免疫吸附法(ELISA)进行VEGF、EPO含量的测定。结果对照组玻璃体中VEGF及EPO浓度分别为(152.8±35.2)pg·mL-1、(74.3±16.5)U·L-1;高度近视组为(81.3±17.5)pg·mL-1、(65.1±13.7)U·L-1;PDR组为(826.3±363.2)pg·mL-1、(649.8±267.9)U·L-1;高度近视合并糖尿病组为(309.4±138.7)pg·mL-1、(156.2±56.7)U·L-1,高度近视合并糖尿病组玻璃体中VEGF、EPO浓度均低于PDR组,差异均有统计学意义(均有P0.05)。结论高度近视对糖尿病患者玻璃体中VEGF和EPO的表达具有抑制作用,VEGF和EPO低表达可能在高度近视阻滞糖尿病视网膜病变的发生发展中发挥作用。
[Abstract]:Objective to investigate the effect of high myopia on diabetic retinopathy by detecting the concentration of vascular endothelial growth factor (VEGF) and erythropoietin (EPO) in vitreous of patients with high myopia and diabetes mellitus. Methods Sixty-two patients (62 eyes) with retinal detachment undergoing vitreous surgery were selected, including 18 eyes of control group, 16 eyes of high myopia group, 15 eyes of proliferative diabetic retinopathy group, 13 eyes of high myopia with diabetes mellitus group. The contents of EPO in vitreous body were determined by double antibody sandwich enzyme-linked immunosorbent assay (Elisa). Results the concentrations of VEGF and EPO in the vitreous of the control group were 152.8 卤35.2)pg mL-1 and 74.3 卤16.5U / L, respectively, while those in the high myopia group were significantly lower than those in the PDR group (826.3 卤363.2)pg ml -1 vs 626.3 卤363.2)pg ml -1 vs 309.4 卤138.7)pg ml -1 vs 156.2 卤56.7U L -1, respectively), and the levels of VEGF-1 EPO in the high myopia group with diabetes mellitus were significantly lower than those in the PDR group (P 0.05). Conclusion High myopia can inhibit the expression of VEGF and EPO in the vitreous of diabetic patients. The low expression of VEGF and EPO may play an important role in the development of diabetic retinopathy.
【作者单位】：郑州大学第一附属医院眼科;
【基金】：河南省医学科技创新人才工程项目[豫卫科(2010)52号]~~
【分类号】：R587.1;R778.11

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