用聚合酶链反应（PCR）法病因诊断慢性结膜炎的研究

发布时间：2018-06-18 23:00

  本文选题：PCR法 + 慢性结膜炎　； 参考：《延边大学》2011年硕士论文

【摘要】：目的：对慢性结膜炎患者结膜囊内分泌物进行DNA提取并加入细菌及腺病毒特异性引物,通过PCR扩增,探讨PCR检测应用于慢性结膜炎病因诊断中的临床意义。 方法：选择慢性感染性结膜炎患者36人(36眼),慢性免疫性结膜炎患者24人(24眼),正常人22人(22眼),两眼共患结膜炎者取较重的一侧。所有受试者结膜囊分泌物提取的DNA分成两部分,分别加入细菌引物及腺病毒引物进行两次PCR扩增,凝胶电泳后在凝胶成像分析系统中观察结果。对于第一组检测人群同时进行结膜囊细菌培养观察结果。 结果：1.慢性感染性结膜炎组(Ⅰ)有14眼扩增出520bp大小条带,阳性率38.89%；4眼扩增出1004bp条带,阳性率11.11%,两者比较有显著性差异(χ2=7.40,P0.05)；2.慢性免疫性结膜炎组(Ⅱ)有3眼扩增出520bp大小条带,阳性率12.5%；1眼扩增出1004bp条带,阳性率4.13%。3.慢性感染性结膜炎组(Ⅰ)细菌培养法有19眼培养出阳性,阳性率为52.78%与PCR检测法比较无显著性差异(χ2=3.2,P0.05)。 结论：1.慢性感染性结膜炎检测细菌感染多于腺病毒感染。2.部分慢性免疫性结膜炎患者可能对细菌或病毒成分或产物致敏,或者检测出细菌为存在于结膜囊内的正常菌群。3.虽然PCR检测法阳性检出率较细菌培养法无明显差异,但对样本要求不高,且经济又时间短,对慢性结膜炎诊断具有良好的应用前景,是一种可供选择的方法。
[Abstract]:Objective: to study the clinical significance of PCR detection in the etiological diagnosis of chronic conjunctivitis by extracting DNA from conjunctival sac secretions of chronic conjunctivitis and adding specific primers of bacteria and adenovirus. Methods: 36 patients with chronic infectious conjunctivitis, 24 patients with chronic immune conjunctivitis and 22 patients with normal conjunctivitis were selected. The DNA extracted from conjunctival sac secretions was divided into two parts. Bacterial primer and adenovirus primer were added for two PCR amplification. The results were observed in the gel imaging analysis system after gel electrophoresis. The results of bacterial culture of conjunctival sac were observed in the first group. The result is 1: 1. In chronic infectious conjunctivitis group (鈪，

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