VIPhybrid对长波长红光诱导近视化作用的影响

发布时间：2018-06-19 16:19

本文选题：长波长光 + 近视　；参考：《眼科新进展》2014年05期

【摘要】：目的观察VIPhybrid(一种非特异性血管活性肠肽拮抗剂)对红光诱导的近视眼生长发育的影响,并探讨巩膜组织中基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)和组织金属蛋白酶抑制因子-2(tissue in-hibitor of metalloproteinase-2,TIMP-2)表达的变化,揭示红光诱导近视形成的可能蛋白通路。方法建立长波长红光诱导的近视模型。将36只豚鼠随机分为3组,前2组右眼分别给予高(A组)、低(B组)浓度VIPhybrid玻璃体内注射饲养在红光中,后一组右眼高浓度VIPhybrid注射在白光中饲养为对照组(C组)。观测3组豚鼠眼球生物学指标、巩膜形态改变、视网膜血管活性肠肽(vasoactive intestinal peptide,VIP)以及巩膜组织中MMP-2/TIMP-2表达情况。结果实验4周后组间比较,红光明显诱导豚鼠眼轴变长,A组左眼眼轴长度为(8.10±0.09)mm、B组左眼为(8.13±0.08)mm,明显长于C组左眼的(7.78±0.07)mm(P0.05);A组右眼眼轴长度为(7.85±0.09)mm,B组右眼为(8.01±0.07)mm,B组抑制眼轴增长的幅度低于A组(P0.05),A组、B组右眼眼轴明显短于左眼(均为P0.05),但仍长于C组(7.76±0.08)mm。A组右眼后巩膜干质量相对较重,视网膜VIP表达明显抑制,后巩膜MMP-2表达降低,TIMP-2表达增高(均为P0.05);B组豚鼠右眼亦发生上述相同变化,但各指标变化幅度均低于A组(均为P0.05);另外与C组相比,A、B组后巩膜干质量、视网膜VIP及巩膜TIMP-2的表达均低于C组,而巩膜MMP-2表达高于C组(均为P0.05)。结论 VIP参与红光诱导的豚鼠近视化作用,阻断VIP可部分延缓近视发展,且有浓度依赖关系。
[Abstract]:Objective to observe the effect of VIP hybrid (a nonspecific vasoactive intestinal peptide antagonist) on the growth and development of myopia induced by red light, and to investigate the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2TIMP-2 (TIMP-2) in scleral tissue. The possible protein pathway of myopia induced by red light was revealed. Methods A long wavelength red light induced myopia model was established. Thirty-six guinea pigs were randomly divided into 3 groups. The first two groups were fed with VIPhybrid intravitreal injection of high concentration VIP-hybrid in the first two groups respectively, and the latter group was fed with high concentration of VIPhybrid in white light as control group (group C). The changes of ocular biology, scleral morphology, vasoactive intestinal peptide and the expression of MMP-2 / TIMP-2 in scleral tissues were observed in the three groups. Results after 4 weeks of experiment, there was a comparison between the two groups. The length of the left eye axis in group A is 8.10 卤0.09 mm and that in group B is 8.13 卤0.08 mm, which is significantly longer than that in group C (7.78 卤0.07 mm) P0.05A group (7.85 卤0.09 mm). The length of right eye axis in group A is 8.01 卤0.07 mm ~ (-1) in group B, which is lower than that in group A (group A) and group B (group B) is lower than that in group A (group A). The axial axis of the eye was shorter than that of the left eye (P 0.05), but still longer than that of group C (7.76 卤0.08)mm.A). The weight of posterior scleral trunk in group C was heavier than that in group C (P 0.05). The expression of VIP in retina was obviously inhibited, and the expression of MMP-2 in posterior sclera was decreased. The expression of TIMP-2 was increased in the posterior sclera (all P0.05%), but the range of changes was lower than that in group A (P 0.05), and the weight of posterior sclera in group A was higher than that in group C. The expression of VIPs and TIMP-2 in retina was lower than that in group C, while MMP-2 expression in sclera was higher than that in group C (P 0.05). Conclusion VIP is involved in the myopia induced by red light in guinea pigs, and blocking VIP can partially delay the development of myopia in a concentration dependent manner.
【作者单位】：首都医科大学附属北京世纪坛医院;
【分类号】：R778.11

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