兔棘阿米巴性角膜炎IL-1β和MCP-1的表达

发布时间：2018-06-21 00:10

本文选题：棘阿米巴角膜炎 + 动物模型　；参考：《福建医科大学》2010年硕士论文

【摘要】： 目的建立一种模拟临床人角膜感染棘阿米巴的动物模型,对其角膜组织损伤的临床表现和组织病理学改变进行观察,同时探讨角膜棘阿米巴原虫感染后角膜炎症细胞因子MCP-1、IL-1β的表达。方法40只新西兰白兔,应用角膜表面镜片术法,即刮除角膜上皮,覆盖角膜植片,二者层间注入棘阿米巴滋养体混悬液,缝合眼睑24h,建立棘阿米巴角膜炎模型,并观察角膜溃疡形态,行角膜HE染色或PAS染色组织病理切片检查,并应用逆转录聚合酶链式反应(RT-PCR)技术,检测不同病程角膜组织中的IL-1β、MCP-l的表达。结果40只兔子右眼均感染棘阿米巴性角膜炎,并经角膜刮片及培养、组织病理切片染色检查证实。感染眼角膜出现水肿,环形基质浸润,角膜新生血管等表现;其病理组织切片上可见基质层内的棘阿米巴滋养体或包囊,伴有中性粒细胞,单核巨噬细胞,嗜酸性粒细胞等的浸润。实验组右眼病变角膜组织中,IL-1β含量于感染后第1天、第3天、第5天均有大量表达,于感染后第3天表达量达到高峰,第7天后呈现逐渐下降趋势,与角膜炎症程度趋势一致(P0.01);MCP-l含量于第1天、第3天、第5天、第7天和第9天均明显升高,于感染后第5天表达量达最高值,以后逐渐下降(P0.01)。结论应用角膜表面镜片法可以成功建立兔棘阿米巴性角膜炎模型,可应用于棘阿米巴角膜炎的免疫学机制研究,IL-1β可能是兔棘阿米巴性角膜炎角膜炎症反应程度的敏感指标;而MCP-1则可能是兔棘阿米巴角膜炎中重要的防御和保护性因素。
[Abstract]:Objective to establish an animal model of Acanthamoeba infection in human cornea and observe the clinical manifestations and histopathological changes of corneal tissue injury. At the same time, the expression of inflammatory cytokine MCP-1and IL-1 尾 in cornea after infection of amoeba cornea was studied. Methods 40 New Zealand white rabbits were treated with keratoscopy, namely, the corneal epithelium was scraped, the corneal graft was covered, the suspension of trophozoites of Acanthamoeba was injected into the two layers, the eyelid was sutured for 24 hours, and the model of Acanthamoeba keratitis was established. The morphology of corneal ulcer was observed and the expression of IL-1 尾 -MCP-l was detected by reverse transcriptase polymerase chain reaction (RT-PCR) technique. Results Acanthoamoeba keratitis was infected in the right eyes of 40 rabbits and was confirmed by corneal scraping and culture. Edema of the infected cornea, infiltration of circular stroma, corneal neovascularization and so on. The trophozoites or cysts of Acanthamoeba in the stroma layer were found in the pathological sections, with neutrophils and mononuclear macrophages. Infiltration of eosinophils, etc. In the experimental group, the expression of IL-1 尾 in the cornea of the right eye lesion reached its peak on the third day after infection, and decreased gradually after 7 days, after infection, and on the first day, the third day and the fifth day after infection, and the expression of IL-1 尾 in the cornea of the experimental group reached its peak on the third day after infection. In accordance with the tendency of corneal inflammation, the content of MCP-l increased significantly on the 1st, 3rd, 5th, 7th and 9th day, and reached the highest level on the 5th day after infection, and then decreased gradually. Conclusion the rabbit model of Acanthamoeba keratitis can be successfully established by using corneal surface lens method, and the immunological mechanism of Acanthamoeba keratitis can be studied. IL-1 尾 may be a sensitive index for the degree of inflammation of rabbit Acanthamoeba cornea. MCP-1 may be an important defensive and protective factor in acanthoamoeba keratitis in rabbits.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R772.21

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