RNA干扰TXR1基因对鼻咽癌细胞耐药的影响

发布时间：2018-06-22 07:54

本文选题：TXR1 + RNA干扰　；参考：《中南大学》2013年博士论文

【摘要】：目的：探索TXR1基因在鼻咽癌紫杉醇耐药中的作用,利用RNA干扰技术抑制鼻咽癌紫杉醇耐药细胞CNE-1/Taxol的TXR1基因表达,同时研究TXR1基因的表达与鼻咽癌紫杉醇耐药的相关性。方法： 1.通过脂质体介导转染siRNA至鼻咽癌紫杉醇耐药细胞株CNE-1/Taxol。 2.利用实时荧光定量RT-PCR. Western-blot方法分别检测RNA干扰前、后的鼻咽癌紫杉醇耐药CNE-1/Taxol细胞株的TXR1基因表达差异。 3.利用MTT实验检测TXR1基因表达抑制后的鼻咽癌紫杉醇耐药细胞CNE-1/Taxol的耐药性变化,观察TXR1基因在紫杉醇耐药过程中的作用。结果： 1.针对TXR1基因的siRNA转染耐药细胞CNE-1/Taxol后,耐药细胞TXR1的mRNA及蛋白表达均明显下调。干扰组和阴性对照组的mRNA表达分别是脂质体组mRNA表达量的0.12±0.02倍、0.97±0.07倍,干扰组分别和阴性对照组、脂质体组比较,差异具有统计学意义(P0.01)。干扰组、阴性对照组的蛋白表达量分别是脂质体组的0.36±0.07、1.02±0.03倍,干扰组与脂质体组、阴性对照组的蛋白表达相比较,差异有统计学意义(P0.01)。 2.TXRl基因表达抑制后,CNE-1/Taxol对紫杉醇的敏感性显著增高,其IC50值降低了2.12倍,从而部分逆转CNE-1/Taxol耐药。结论： 1.特异性siRNA分子能有效的抑制鼻咽癌紫杉醇耐药细胞的TXR1基因表达。 2.运用RNA干扰技术抑制TXR1基因可能部分逆转人鼻咽癌紫杉醇耐药细胞CNE-1/Taxol的耐药性。TXR1基因可能成为逆转鼻咽癌细胞紫杉醇耐药及肿瘤治疗的靶分子。图7幅,表2个,参考文献53篇
[Abstract]:Aim: to investigate the role of TXR1 gene in the resistance of nasopharyngeal carcinoma (NPC) to paclitaxel, and to investigate the relationship between TXR1 gene expression and taxol resistance in nasopharyngeal carcinoma (NPC) cells CNE-1 / Taxol by RNA interference. Methods: 1. Transfection of siRNA into CNE-1 / Taxol-2. 2 by liposome mediated transfection of siRNA into CNE-1 / Taxol- resistant nasopharyngeal carcinoma cell line. Real-time fluorescence quantitative RT-PCR was used. Western-blot method was used to detect the difference of TXR1 gene expression in CNE-1 / Taxol cell line before and after RNA interference. The drug resistance of CNE-1 / Taxol cells after TXR1 gene expression inhibition was detected by MTT assay, and the role of TXR1 gene in taxol resistance was observed. Results: 1. After transfection of siRNA targeting TXR1 gene into CNE-1 / Taxol, the mRNA and protein expression of TXR1 was down-regulated. The mRNA expression of interference group and negative control group was 0.12 卤0.02 times 0.97 卤0.07 times of that of liposome group, respectively. The difference between interference group and negative control group was statistically significant (P0.01). The protein expression in interference group and negative control group was 0.36 卤0.07 卤1.02 卤0.03 times of that in liposome group, and the protein expression in interference group was higher than that in liposome group and negative control group. 2. The sensitivity of CNE-1 / Taxol to paclitaxel was significantly increased after the inhibition of TXRl gene expression, and the IC50 value of CNE-1 / Taxol decreased 2.12 times, which partially reversed the resistance of CNE-1 / Taxol. Conclusion: 1. Specific siRNA molecules can effectively inhibit the expression of TXR1 gene in paclitaxel-resistant nasopharyngeal carcinoma cells. 2. Inhibition of TXR1 gene by RNA interference may partially reverse the drug resistance of CNE-1 / Taxol cell line CNE-1 / Taxol. TXR1 gene may be a target molecule for reversing taxol resistance and tumor therapy of nasopharyngeal carcinoma cell line CNE-1 / Taxol. 7 figures, 2 tables, 53 references
【学位授予单位】：中南大学
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R739.63

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