鼻咽癌中VEGF及其受体EGFR的表达及相关性的初步研究

发布时间：2018-06-23 09:24

  本文选题：VEGF + EGFR　； 参考：《浙江大学》2011年硕士论文

【摘要】：背景和目的： 鼻咽癌是头颈部最常见的恶性肿瘤,世界卫生组织统计大约有80%的鼻咽癌发生与我国。VEGF(血管内皮生长因子)是目前已知最具活性的促血管生成因子之一,能促进血管内皮增殖,增加血管通透性,为血管内皮细胞的生长、迁移提供基质条件,并且具有抑制肿瘤细胞凋亡的重要作用。EGFR(血管内皮生长因子受体)对VEGF具有高度亲和性,二者特异结合后,促发细胞分裂信号,引起细胞增殖,EGFR表达异常,可使正常细胞转化成肿瘤细胞,并增加肿瘤的侵袭和转移能力。本研究运用免疫组化检测57例鼻咽癌组织中的VEGF、EGFR的表达,27例鼻咽部慢性炎症组织中的VEGF、EGFR的表达,研究VEGF和EGFR表达水平与临床资料之间以及相互之间的关系。 材料和方法： 2009年-2010年在我院初诊确定为鼻咽癌的鼻咽部活检标本57例。其中男性44例,女性13例；年龄为17-74岁；病理分型为非角化性癌未分化型19例,非角化性癌分化型30例,低分化鳞状细胞癌8例。根据2002年AJCC标准57例中T1 27例,T2 17例,T3 8例,T4 5例；N0 16例,N1 20例,N2 19例,N3 2例；Ⅰ期13例,Ⅱ期13例,Ⅲ期19例,Ⅳ期12例。27例鼻咽部慢性炎症常规石蜡切片标本由我院病理科同期鼻咽部活检标本中选取,由HE染色确诊为鼻咽部黏膜慢性炎。其中男性16例,女性11例,作为对照组。所有标本离体后立即用10%中性甲醛固定,常规石蜡包埋,石蜡切片2μm厚连续切片,采用Envision TM二步法检测VEGF、EGFR的表达,用SPSS13.0软件对实验数据进行统计。 结果： 1.VEGF、EGFR在鼻咽癌组织中均有阳性表达。 2. VEGF在鼻咽癌中的表达与淋巴结转移及临床分期有显著相关性(P0.05),但和患者的年龄、性别、病理类型无相关性(P0.05)。 3. EGFR的表达和鼻咽癌的颈部淋巴结转移存在显著相关性(P0.05),但和患者的年龄、性别、病理类型、临床分期无相关性(P0.05)。 4. EGFR在鼻咽癌中的表达与VEGF呈正相关(P0.01) 结论： 1.VEGF及EGFR在鼻咽癌组织中均有广泛的表达。 2.VEGF的表达和鼻咽癌的颈部淋巴结转移与临床分期存在显著相关性,但和患者的年龄、性别、病理类型无相关性。 3.EGFR的表达和鼻咽癌的颈部淋巴结转移存在显著相关性,但和患者的年龄、性别、病理类型、临床分期无相关性。 4. EGFR在鼻咽癌中的表达与VEGF呈正相关,两者在鼻咽癌致病中可能有共同或相关途径存在。
[Abstract]:Background and objective: nasopharyngeal carcinoma is the most common malignant tumor in the head and neck. The World Health Organization (WHO) estimates that about 80% of nasopharyngeal carcinogenesis and vascular endothelial growth factor (VEGF) is one of the most active angiogenic factors known at present, which can promote vascular endothelial proliferation and increase vascular permeability. It provides the matrix condition for the growth and migration of vascular endothelial cells, and has an important role in inhibiting the apoptosis of tumor cells. EGFR (vascular endothelial growth factor receptor) has high affinity for VEGF. The abnormal expression of EGFR could induce the transformation of normal cells into tumor cells and increase the ability of tumor invasion and metastasis. In this study, the expression of VEGF and EGFR in 57 cases of nasopharyngeal carcinoma and 27 cases of chronic inflammation of nasopharynx were detected by immunohistochemistry, and the relationship between the expression of VEGF and EGFR and clinical data was studied. Materials and methods: from 2009 to 2010, 57 nasopharyngeal biopsy specimens were first diagnosed as nasopharyngeal carcinoma in our hospital. Among them, 44 cases were male, 13 cases were female, the age was 17-74 years old, 19 cases were undifferentiated type of non-keratinizing carcinoma, 30 cases were non-keratinizing carcinoma, and 8 cases were poorly differentiated squamous cell carcinoma. According to the AJCC standard of 2002, there were 27 cases of T 1, 17 cases of T 2, 8 cases of T 3, 45 cases of T 3, 15 cases of N 1, 20 cases of N 2, 19 cases of N 2, 13 cases of stage 鈪，

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