Livin、PTEN及MMP-9在视网膜母细胞瘤中的表达及相关性研究

发布时间：2018-06-23 16:00

本文选题：视网膜母细胞瘤 + Livin　；参考：《眼科新进展》2014年02期

【摘要】：目的研究Livin、PTEN及基质金属蛋白酶-9(matrix metauoproteinase-9,MMP-9)在视网膜母细胞瘤(retinoblastoma,RB)中的表达和临床意义,并探讨三者之间的相关性。方法对56例RB组织标本进行Livin、PTEN及MMP-9蛋白表达的免疫组织化学染色,以正常视网膜组织标本10例作为正常对照,采用免疫组织化学法检测56例RB组织中及10l 例正常视网膜组织中的Livin、PTEN及MMP-9的阳性表达情况,探讨3种因子在正常视网膜及RB中的表达及相关性。结果Livin蛋白在RB中阳性表达,阳性表达率为57.1%,但其在正常视网膜组织中不表达(P0.05);PTEN蛋白在正常视网膜表达,阳性率达100.0%,而在RB组织中表达率仅为55.4%,差异有统计学意义(P0.05);RB组织中MMP-9阳性表达率高达50.0%,在正常视网膜组织中不表达,两者比较差异有统计学意义(P0.05)。PTEN、Livin及MMP-9的蛋白表达与RB分期、病理学分型及伴视神经浸润有关(均为P0.05),与性别及年龄无关(均为P0.05)。PTEN和Livin的表达呈显著负相关(r=-0.370,P0.05),与MMP-9的表达也呈显著负相关(r=-0.310,P0.05);而Livin和MMP-9在RB中的表达呈显著正相关(r=0.450,P0.01)。PTEN表达与MMP-9、Livin共表达存在负相关(r=-0.542,P0.01)。结论 Livin、PTEN及MMP-9可作为判断RB恶性程度和预后的指标,在RB的早期诊断中有重要作用。
[Abstract]:Objective to investigate the expression and clinical significance of Livinopsin (PTEN) and matrix metalloproteinase-9 (MMP-9) in retinoblastoma (RB), and to explore the correlation between them. Methods Immunohistochemical staining of PTEN and MMP-9 was performed in 56 cases of RB tissues and 10 cases of normal retina tissues as control. Immunohistochemical method was used to detect the positive expression of Livinn PTEN and MMP-9 in 56 RB tissues and 101 normal retina tissues, and to explore the expression and correlation of three factors in normal retina and RB. Results the positive expression rate of Livin protein in RB was 57.1%, but it was not expressed in normal retina tissue (P0.05) PTEN protein was expressed in normal retina. The positive rate of MMP-9 in RB tissues was 55.4, and the positive rate of MMP-9 in RB tissues was 50.0.The positive rate of MMP-9 in RB tissues was as high as 50.0g, but not in normal retina tissues. The difference between them was statistically significant (P0.05). The protein expression of PTENU Livin and MMP-9 and the stages of RB were significantly higher than those in normal retina tissues (P0.05), and the positive rate of MMP-9 in RB tissues was significantly higher than that in normal retina tissues (P0.05). The expression of PTEN and Livin was negatively correlated with the expression of PTEN and Livin (r-0.370, P0.05), and the expression of MMP-9 was negatively correlated with the expression of MMP-9 (r-0.310, P0.05), while the expression of Livin and MMP-9 in RB was significantly positive. There was a negative correlation between the expression of PTEN and the expression of MMP-9 livin (r-0.542p0.01). Conclusion Livinn PTEN and MMP-9 can be used as indicators to judge the malignancy and prognosis of RB and play an important role in the early diagnosis of RB.
【作者单位】：甘肃省康复中心医院;兰州大学基础医学院病理学研究所;兰州大学石化总医院;
【分类号】：R739.7

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