Toll样受体2和Toll样受体4在慢性鼻-鼻窦炎中的表达及意义

发布时间：2018-06-27 10:35

本文选题：慢性鼻-鼻窦炎 + 天然免疫　；参考：《临床耳鼻咽喉头颈外科杂志》2014年17期

【摘要】：目的:通过检测天然免疫因子Toll样受体2(TLR2)和Toll样受体4(TLR4)在慢性鼻-鼻窦炎不同临床分型以及对照组中的表达情况,探讨TLR在慢性鼻-鼻窦炎发病机制中的作用。方法:采用免疫组织化学方法分别检测TLR2和TLR4在慢性鼻-鼻窦炎伴鼻息肉组(A组,21例),慢性鼻-鼻窦炎无鼻息肉组(B组,15例),复发性慢性鼻-鼻窦炎伴鼻息肉组(C组,11例)及对照组(D组,13例)中的表达情况。显微镜下计数阳性细胞表达情况,等级资料采用Mann-Whitney U进行统计分析,组间用单因素的方差进行统计分析。结果:1TLR2和TLR4有相同的表达特点,主要表达部位集中在上皮基膜全层、腺体细胞的细胞质及极少数炎性细胞,如单核细胞和浆细胞的细胞质,偶有不明细胞的细胞核阳性表达;2A、B、C、D组鼻腔黏膜组织中TLR2的表达分别为10.18±16.80、27.00±12.07、7.55±14.69、9.40±17.83,A、C、D组与B组比较,均差异有统计学意义(均P0.05);3A、B、C、D组鼻腔黏膜组织中TLR4的表达分别为11.30±19.22、36.02±17.75、8.51±9.22、7.75±4.49,A、C、D组与B组比较,均差异有统计学意义(均P0.05)。结论:鼻腔黏膜上皮能产生天然免疫因子TLR2和TLR4;TLR2和TLR4在慢性鼻-鼻窦炎各临床表型中的差异性表达提示其在慢性鼻-鼻窦炎的发病中发挥了一定作用。
[Abstract]:Objective: to investigate the role of TLR in the pathogenesis of chronic rhinosinusitis by detecting the expression of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) in different clinical types of chronic rhinosinusitis and control group. Methods: immunohistochemical method was used to detect TLR2 and TLR4 in 21 patients with chronic rhinosinusitis with nasal polyps (group A), 15 patients with chronic rhinosinusitis without nasal polyps (group B) and 15 patients with recurrent chronic rhinosinusitis with nasal polyps. In group C (n = 11) and control group (group D, n = 13). The expression of positive cells was counted under microscope. Mann-Whitney U was used to analyze the grade data and the variance of single factor was used for statistical analysis among groups. Results the expression of TLR2 and TLR4 was the same, mainly in the whole layer of epithelial basement membrane, the cytoplasm of glandular cells and the cytoplasm of a few inflammatory cells, such as monocytes and plasma cells. The positive expression of TLR2 in nasal mucosa of group D was 10.18 卤16.80 卤27.00 卤12.07 卤7.55 卤14.69 卤9.40 卤17.83C (P 0.05). The expression of TLR4 in nasal mucosa of group D was 11.30 卤19.225.02 卤17.77.51 卤9.227.75 卤4.49.75 卤4.49.9. the expression of TLR4 in nasal mucosa of group D was significantly higher than that of group B (P 0.05). The difference was statistically significant (P0.05). Conclusion: the differential expression of TLR2 and TLR4TLR2 and TLR4 in the clinical phenotypes of chronic rhinosinusitis suggests that TLR2 and TLR4 may play a role in the pathogenesis of chronic rhinosinusitis.
【作者单位】：哈尔滨医科大学附属第二医院耳鼻咽喉头颈外科;海林市人民医院耳鼻咽喉科;
【基金】：黑龙江省教育厅科技面上项目(No:12541272)
【分类号】：R765.41

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