雌激素受体α和β在人类喉癌中的表达及其意义

发布时间：2018-06-28 07:15

本文选题：喉癌 + 雌激素受体亚型　；参考：《辽宁医学院》2011年硕士论文

【摘要】：目的喉癌是呼吸道的恶性肿瘤之一,男性较女性多见,约为7～10:1,这提示性别因素在发病机制中起到作用。雌激素通过雌激素受体α和β(ERα,ERβ)影响着激素依赖性器官癌症的病理过程。最新研究表明,ERα具有促进细胞增殖的作用,ERβ具有抑制细胞增殖的作用。然而,ERα、ERβ在喉癌中表达情况并不明确,本研究旨在探讨ERα、ERβ在喉癌及癌旁组织中的表达情况及其与喉癌生物学特征的关系。方法采用Picture MT-Pv9000免疫组织化学技术检测72例喉癌及8例癌旁组织中雌激素受体α和β的表达。HE染色鉴定组织性质,Western印迹法及RT-PCR法鉴定80例标本中9例ERα、ERβ的表达。实验数据用SPSS13.0统计分析软件处理,采用χ2检验进行分析。结果 ERα主要表达在细胞核内,个别以细胞质中表达为主。ERα在喉癌组织中表达阳性率为43.06%,而癌旁组织无表达,差异有统计学意义(P0.05)。 ERβ主要表达在细胞核内,个别以细胞质中表达为主。ERβ在喉癌组织和癌旁组织表达阳性率分别为27.78%和25%,差异无统计学意义(P0.05)。 ERα在喉癌III～IV期的表达率强于I～II期,有淋巴结转移者表达率强于无淋巴结转移者,ERα在喉癌阳性表达率与临床分期、淋巴结转移呈正相关,差异有统计学意义(P0.05),ERα在喉癌不同病理分型中表达的差异有统计学意义,ERα在喉癌中的阳性表达率有随病理分级升高而增加的趋势。ERβ则与临床分期、淋巴结转移呈负相关,差异有统计学意义(P0.05)。ERβ在喉癌不同病理分型中表达的差异有统计学意义,ERβ在喉癌中的阳性表达率有随病理分级升高而减少的趋势。ERα、ERβ表达率与性别、肿瘤部位无明显相关性。 Western印迹法示ERα蛋白在喉癌组织中有表达,癌旁组织中无表达,差异有统计学意义(P0.05),ERβ蛋白在喉癌组织中有表达,癌旁组织中也有表达,差异无统计学意义(P0.05),这与免疫组织化学结果基本相符,同时在55KD和43KD之间出现了2个条带分别为ERβWT、ERβE5SV。 RT-PCR检测结果与Western印迹法、免疫组化结果基本相符。结论 ERα和ERβ在喉癌中均有表达,以ERα表达占优势。 ERα在喉癌中有表达,在癌旁组织中无表达,ERα的表达与喉癌临床分期、淋巴结转移呈正相关,ERα在喉癌中的阳性表达率有随病理分级升高而增加的趋势。 ERβ在喉癌及癌旁组织中均有表达,ERβ的表达与喉癌临床分期、淋巴结转移呈负相关,ERβ在喉癌中的阳性表达率有随病理分级升高而减少的趋势。 ERα和ERβ的表达与患者肿瘤部位、性别无关。
[Abstract]:Objective laryngeal carcinoma is one of the malignant tumors of respiratory tract, which is more common in men than in women, and is about 710: 1, which suggests that sex plays an important role in the pathogenesis. Estrogen affects the pathological process of hormone dependent organ cancer through estrogen receptors 伪 and 尾 (ER 伪 and ER 尾). Recent studies have shown that ER 伪 can promote cell proliferation and ER 尾 can inhibit cell proliferation. However, the expression of ER 伪 and ER 尾 in laryngeal carcinoma is not clear. The purpose of this study was to investigate the expression of ER 伪 and ER 尾 in laryngeal carcinoma and its relationship with the biological characteristics of laryngeal carcinoma. Methods Picture MT-Pv9000 immunohistochemical technique was used to detect the expression of estrogen receptor 伪 and 尾 in 72 cases of laryngeal carcinoma and 8 cases of adjacent tissues. The experimental data were analyzed by SPSS 13.0 statistical analysis software and 蠂 2 test. Results ER 伪 was mainly expressed in the nucleus. The positive rate of ER 伪 in laryngeal carcinoma tissues was 43.06, but there was no expression in paracancerous tissues (P0.05). The expression of ER 尾 was mainly in the nucleus. The positive rates of ER 尾 expression in laryngeal carcinoma tissues and adjacent tissues were 27.78% and 25% respectively (P0.05). The expression rate of ER 伪 in stage IIIIV of laryngeal carcinoma was higher than that in stage II of laryngeal carcinoma. The positive expression rate of ER 伪 in laryngeal carcinoma with lymph node metastasis was higher than that without lymph node metastasis and had positive correlation with clinical stage and lymph node metastasis. There was a significant difference in the expression of ER 伪 in different pathological types of laryngeal carcinoma. The positive expression rate of ER 伪 in laryngeal carcinoma increased with the increase of pathological grade. There was a negative correlation between ER 尾 and clinical stage and lymph node metastasis. There were significant differences in the expression of ER 尾 in different pathological types of laryngeal carcinoma (P0.05). The positive expression rate of ER 尾 in laryngeal carcinoma decreased with the increase of pathological grade. The expression rate of ER 伪 and ER 尾 decreased with the increase of pathological grade. Western blot showed that ER 伪 protein was expressed in laryngeal carcinoma tissues, but not in paracancerous tissues. The difference was statistically significant (P0.05) the expression of ER 尾 protein in laryngeal carcinoma tissues and paracancerous tissues was also found. The difference was not statistically significant (P0.05), which was basically consistent with the immunohistochemical results. At the same time, there were two bands between 55KD and 43KD, which were ER 尾 WTER 尾 E5SV.The results of RT-PCR and Western blot showed that the results of RT-PCR were basically consistent with those of Western blotting. Conclusion ER 伪 and ER 尾 are expressed in laryngeal carcinoma, the expression of ER 伪 is dominant in laryngeal carcinoma, ER 伪 is expressed in laryngeal carcinoma, no expression of ER 伪 is found in paracancerous tissue, and the expression of ER 伪 is associated with the clinical stage of laryngeal carcinoma. The positive expression rate of ER 伪 in laryngeal carcinoma was positively correlated with lymph node metastasis. The expression of ER 尾 in laryngeal carcinoma and adjacent tissues was increased with the increase of pathological grade, and the expression of ER 尾 was correlated with the clinical stage of laryngeal carcinoma. The positive expression rate of ER 尾 in laryngeal carcinoma decreased with the increase of pathological grade, but the expression of ER 伪 and ER 尾 was not related to tumor location and sex.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.65

【相似文献】