溴芬酸钠对人晶状体上皮细胞增殖、迁移的效应研究

发布时间：2018-06-30 01:46

本文选题：后发性白内障 + 溴芬酸钠　；参考：《浙江大学》2014年硕士论文

【摘要】：目的：后发性白内障是现代白内障术后常见的长期并发症。本研究拟探讨溴芬酸钠对体外培养的人晶状体上皮细胞增殖、迁移的效应研究,评价其在治疗后发性白内障的作用。方法：将人晶状体上皮细胞用不同浓度的TNF-α (5、10、15、20、25ng/ml)培养24小时,进一步选择合适浓度的TNF-α来建立眼内炎症模型。用MTT比色法、transwell方法来评估澳芬酸钠在TNF-α诱导下对HLECs增殖、迁移的效应。结果：不同浓度的TNF-α (5、10、15、20、25ng/ml)培养人晶状体上皮细胞24小时后细胞增殖率分别为21.1%、60.9%、62.9%、64.6%、66.6%。统计学结果显示,空白对照组、TNF-α5ng/ml组分别与TNF-α(10、15、20、25) ng/ml各组相比,P0.01;而TNF-α10ng/ml组与TNF-α(15、20、25)ng/ml各组相比,P0.05,最终选用TNF-α10ng/ml建立并模拟眼内炎症模型。在相同浓度TNF-α诱导下,不同浓度的溴芬酸钠作用于人晶状体上皮细胞24小时。从MTT法测得的结果可知,在TNF-a lOng/ml诱导情况下,BS0ug/ml组与BS (20、40、60、80) ug/ml各组之间相比,P0.01；即在炎症条件下,溴芬酸钠能明显抑制TNF-α诱导的HLECs增殖效应,且呈剂量-效应关系。从transwell法测得的结果可知,在TNF-alOng/ml诱导情况下,BS Oug/ml组与BS(40、60、80)ug/ml各组之间相比,P0.01；即在炎症条件下,溴芬酸钠能明显抑制TNF-a诱导的HLECs迁移效应,且呈剂量-效应关系。结论：溴芬酸钠能明显抑制TNF-α诱导状态下的HLECs增殖和迁移,并呈剂量-效应关系。因此有望成为防治后发性白内障的有效药物。
[Abstract]:Objective: posterior cataract is a common long-term complication after modern cataract surgery. The aim of this study was to investigate the effects of bromofenac sodium on proliferation and migration of cultured human lens epithelial cells (LECs) and evaluate its effect on the treatment of post-cataract. Methods: human lens epithelial cells were cultured with different concentrations of TNF- 伪 for 24 hours. MTT colorimetric assay was used to evaluate the effect of oxafenac sodium on proliferation and migration of HLECs induced by TNF- 伪. Results: the proliferation rate of human lens epithelial cells cultured with different concentrations of TNF- 伪 (5 ~ 10 ~ 10 ~ 15 ~ 20 ~ 20 ~ 25 ng / ml) for 24 hours was 21.1% and 60.9% respectively. The statistical results showed that TNF- 伪 5ng/ml group and TNF- 伪 10ng/ml group were respectively compared with TNF- 伪 (10 ~ 1515 ~ 20 ~ 25) ng/ml group (P 0.01) and TNF- 伪 10ng/ml group compared with TNF- 伪 (15 ~ 20 ~ (25) ng/ml group (P < 0.05). Finally, TNF- 伪 10ng/ml was used to establish and simulate intraocular inflammation model. Under the same concentration of TNF- 伪, different concentrations of bromofenac sodium acted on human lens epithelial cells for 24 hours. The results of MTT assay showed that BS0ugrml group was significantly inhibited by TNF- 伪 -induced proliferation of HLECs in a dose-effect manner, compared with that of BS (200.40,6080) ug/ml group under the condition of inflammation, which was induced by TNF-a lOng / ml, and the results showed that BS0ugrml / ml group could significantly inhibit TNF- 伪 -induced proliferation of HLECs in a dose-effect manner. The results obtained by transwell method showed that under the induction of TNF-alOng / ml, there was a dose-effect relationship between BS Oug / ml group and BS (40,6080) ug/ml group, that is, bromofenac sodium could significantly inhibit TNF-a induced HL-ECs migration in a dose-dependent manner under inflammatory conditions. Conclusion: bromofenac sodium can inhibit the proliferation and migration of HLECs induced by TNF- 伪 in a dose-dependent manner. Therefore, it is expected to be an effective drug for the prevention and treatment of posterior cataract.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R776.1

【共引文献】