庆大霉素对内毛细胞带状突触CaV1.3钙离子通道蛋白表达的影响

发布时间：2018-07-03 16:56

本文选题：庆大霉素 + 耳蜗　；参考：《临床耳鼻咽喉头颈外科杂志》2014年04期

【摘要】：目的:研究庆大霉素对C57BL/6J小鼠听力及耳蜗内毛细胞带状突触CaV1.3钙通道蛋白数量的影响,探讨听力损失与其剂量相关性变化之间的关系及意义。方法:采用固定剂量[100mg/(kg·d)]的氨基苷类抗生素(庆大霉素)对C57BL/6J小鼠每日进行腹腔注射造模,分别在注射后的第7天、第14天、第28天(注射14d停药14d)应用ABR分别测得小鼠的听力。免疫荧光方法观察耳蜗基膜内毛细胞带状突触CaV1.3钙通道蛋白的分布和表达(其中0天为空白对照组),内毛细胞突触前后膜分别采用CtbP2和CaV1.3进行免疫荧光标记。结果:随着注射药物天数的增长ABR反应阈值明显提高,所有小鼠内毛细胞数量、形态均无明显变化,但内毛细胞带状突触CaV1.3钙离子通道蛋白量的表达存在显著差异(P0.01)。CaV1.3钙离子通道蛋白在庆大霉素腹腔注射CaV1.3/CtBP2表达数量在第7天时略有增加,在第14天时其表达数量明显减少,第28天与第14天比较CaV1.3/CtBP2表达数量有所增加,但低于正常。结论:在氨基苷类抗生素毒性环境中耳蜗内毛细胞CaV1.3蛋白表现为先增加后减少再增加的趋势,提示内毛细胞带状突触CaV1.3蛋白数量的增加可能存在对这类药物毒性的代偿作用,而随着药物毒性作用时间的增加,这种失代偿作用表现为听力下降,可能是损伤听力的机制之一。
[Abstract]:Aim: to study the effects of gentamicin on the number of calcium channel protein (CaV1.3) in the band synapses of C57BL / 6J mice and to explore the relationship between hearing loss and its dose-dependent changes. Methods: C57BL / 6J mice were intraperitoneally injected with gentamicin at a fixed dose of [100mg/ (kg d)]. The hearing of C57BL / 6J mice was measured on the 7th, 14th and 28th day after injection. The distribution and expression of CaV1.3 calcium channel protein (CaV1.3) in hair cells of cochlear basal membrane were observed by immunofluorescence method. CtbP2 and CaV1.3 were used to label the synaptic membrane of the inner hair cell before and after treatment with CtbP2 and CaV1.3, respectively. Results: with the increase of the days of injection, the ABR reaction threshold increased significantly, and the number and morphology of hair cells in all mice did not change significantly. However, there was a significant difference in the expression of CaV1.3 calcium channel protein in the inner hair cells (P0.01) .CaV1.3 calcium channel protein in gentamicin intraperitoneal injection of CaV1.3 / CtBP2 increased slightly on the 7th day, and decreased significantly on the 14th day. On the 28th day, compared with the 14th day, the expression of CaV1.3 / CtBP2 was increased, but lower than that of the normal. Conclusion: in the toxic environment of aminoside antibiotics, the expression of CaV1.3 protein in cochlear hair cells increased first and then decreased, suggesting that the increase in the number of band synaptic CaV1.3 protein in the inner hair cells may have a compensatory effect on the toxicity of these drugs. With the increase of the time of drug toxicity, the decompensation may be one of the mechanisms of hearing loss.
【作者单位】：锦州市中心医院耳鼻咽喉头颈外科;辽宁医学院附属第一医院耳鼻咽喉头颈外科;中国人民解放军总医院耳鼻咽喉头颈外科;
【分类号】：R764

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