软叶针葵花粉疫苗治疗小鼠过敏性鼻炎的效果和机制

发布时间：2018-07-08 14:40

本文选题：软叶针葵花粉 + 过敏性鼻炎　；参考：《细胞与分子免疫学杂志》2014年02期

【摘要】：目的建立小鼠过敏性鼻炎模型,用重组软叶针葵花粉profilin疫苗免疫预防治疗,观察过敏性鼻炎小鼠的病理变化,探讨该疫苗对过敏性鼻炎治疗效果及可能机制。方法用腹腔注射法建立BALB/c小鼠软叶针葵花粉过敏性鼻炎模型,皮下注射过敏原疫苗进行预防治疗。通过小鼠无创肺功能仪检测小鼠治疗前后气道高反应性、间接ELISA检测血清中特异性抗体(IgE和IgG2a)、细胞因子,透射电镜观察鼻黏膜组织超微结构变化。结果疫苗特异性免疫治疗后,小鼠鼻部症状和气道高反应性减轻,其血清中特异性IgG2a水平升高、IgE水平降低,培养脾细胞上清中INF-γ、IL-10含量增加,IL-4生成减少。结论重组软叶针葵花粉profilin作为疫苗对花粉过敏性鼻炎有一定的疗效,可能与促使Th2向Th1转换,调节辅助性T细胞的平衡有关。
[Abstract]:Objective to establish an allergic rhinitis model in mice, and to observe the pathological changes of mice with allergic rhinitis by immunotherapy with recombinant soft leaf needle sunflower powder profilin vaccine, and to explore the therapeutic effect and possible mechanism of this vaccine on allergic rhinitis. Methods the allergic rhinitis model of sunflower powder of soft leaf needle in BALB / c mice was established by intraperitoneal injection, and subcutaneous injection of allergen vaccine was used to prevent rhinitis. The airway hyperresponsiveness of mice before and after treatment was detected by non-invasive pulmonary function analyzer, the specific antibodies (IgE and IgG2a) in serum, cytokines and ultrastructure of nasal mucosa were detected by indirect Elisa. Results after specific immunotherapy, the nasal symptoms and airway hyperresponsiveness of mice were alleviated, the level of serum specific IgG2a increased and the level of IgE decreased, while the content of INF- 纬 IL-10 in the supernatant of spleen cells increased and the production of IL-4 decreased. Conclusion Recombinant soft leaf sunflower powder profilin is effective in the treatment of pollen allergic rhinitis, which may be related to the transformation of Th2 to Th1 and the regulation of the balance of helper T cells.
【作者单位】：深圳大学生命科学学院;深圳大学医学院过敏反应与免疫学研究所;南昌大学基础医学院免疫学教研室;
【基金】：国家自然科学基金(30070702;811160129) 广东省高等学校国际暨港澳台科技合作创新平台项目(2012gjhz0009) 深圳市重点实验室组建项目(SW201110010)
【分类号】：R765.21

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