鬼针草滴眼液预防去势雄兔干眼症的实验研究

发布时间：2018-07-08 16:02

本文选题：鬼针草滴眼液 + 干眼　；参考：《眼科新进展》2014年12期

【摘要】：目的研究鬼针草滴眼液对雄兔去势所致干眼症的预防作用。方法选取新西兰白兔24只(24眼,均为右眼),制作去势雄兔干眼症模型,模型制作后即随机分为A、B两组(各12眼):实验组(A组)使用鬼针草滴眼液滴眼,对照组(B组)使用磷酸盐缓冲液(phosphate buffered saline,PBS)滴眼。另选取2只兔作为正常对照分别于治疗前和治疗后1周、2周、1个月及2个月进行Schirmer I试验(Schirmer I test,SIT)检查、角膜荧光素染色(fluorescein,FL),并进行泪液总蛋白含量、淀粉酶活性、乳铁蛋白、溶菌酶含量检测及角膜共聚焦显微镜扫描。结果治疗前A、B两组SIT、FL评分、泪液总蛋白量、乳铁蛋白、溶菌酶及淀粉酶活性相比,差异均无统计学意义(均为P0.05);治疗1周、2周、1个月及2个月后,B组SIT、FL评分较治疗前均稍有恶化,差异均有统计学意义(均为P0.05),而泪液总蛋白量、乳铁蛋白、溶菌酶及淀粉酶活性与治疗前相比差异均无统计学意义(均为P0.05);A组SIT、FL评分、泪液总蛋白量、乳铁蛋白、溶菌酶及淀粉酶活性均有不同程度改变,差异均有统计学意义(均为P0.05);A、B两组各时间点SIT、FL评分、泪液总蛋白量、乳铁蛋白、溶菌酶及淀粉酶活性相比,差异均有统计学意义(均为P0.05)。治疗2个月后,A组上皮基底细胞和炎症细胞密度分别为(4262±268)mm-2和(312±103)mm-2,而B组上皮基底细胞和炎症细胞密度分别为(3106±237)mm-2和(32±23)mm-2,两组比较,差异均有统计学意义(均为P0.05)。结论鬼针草滴眼液能有效预防雄激素水平降低所致兔干眼症。
[Abstract]:Objective to study the preventive effect of nitifera eye drops on dry eye syndrome induced by castration in male rabbits. Methods Twenty-four New Zealand white rabbits (24 eyes, all right eyes) were selected to make the dry eye model of ovariectomized male rabbits. After the establishment of the model, they were randomly divided into two groups: group A (group A) and group A (group A). The eyes of the control group (group B) were treated with phosphate buffer solution (phosphate buffered Saline PBS). Schirmer I test (Schirmer I testSIT), fluorescein FL (fluorescein FL) were performed before treatment and 1 week, 1 month and 2 months after treatment in two rabbits as normal control, and the contents of total protein, amylase activity and lactoferrin in tears were measured. Lysozyme content and corneal confocal microscope scanning. Results before treatment, the scores of SITF, total protein in tear, lactoferrin, lysozyme and amylase activity were compared between the two groups. The scores of SITF in group B were slightly worse than those before treatment (P0.05), while the total protein content of tear, lactoferrin, and lactoferrin in group B were significantly worse than those before treatment (P0.05), the difference was not significant (P0.05), and that of lactoferrin, lactoferrin and lactoferrin in group B was significantly higher than that in group B (P0.05). There was no significant difference in lysozyme and amylase activity between before and after treatment (P0.05). In group A, the total protein content, lactoferrin, lysozyme and amylase activity changed in different degrees. There were significant differences in SITFL score, total protein content, lactoferrin, lysozyme and amylase activity between the two groups (P0.05). After 2 months of treatment, the density of epithelial basal cells and inflammatory cells in group A was (4262 卤26.8) mm-2 and (312 卤103mm ~ (-2) respectively, while that of epithelial basal cells and inflammatory cells in group B was (3106 卤237) mm-2 and (32 卤23) mm ~ (-2), respectively. There was significant difference between the two groups (P0.05). Conclusion Herba nitifera eyedrops can effectively prevent dry eye syndrome caused by the decrease of androgen level.
【作者单位】：南昌大学第一附属医院眼科;永新人民医院眼科;厦门大学附属厦门眼科中心;
【基金】：国家自然科学基金(编号:m81160118、81400372、81060063、81170823、81100648、81100649) 全国临床医药专项基金z(编号:L2012052) 江西省青年科学基金(编号:020114BAB215036) 江西省科技支撑计划项目(编号:20111BBG70026-2) 江西省卫生厅中医药科研项目(编号:2012A087) 江西省教育厅科技计划项目(编号:GJJ14170)~~
【分类号】：R777

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