眼内植入型伏立康唑缓释系统对烟曲霉菌性眼内炎影响的实验研究

发布时间：2018-07-09 22:44

  本文选题：伏立康唑 + 缓释系统　； 参考：《青岛大学》2010年硕士论文

【摘要】： 目的评价玻璃体腔内植入伏立康唑缓释系统(VCZ-DDS)对兔烟曲霉菌性眼内炎的影响,探索最佳给药剂量,探讨VCZ-DDS的体内释放规律。 方法伏立康唑以PLGA为载体制成VCZ-DDS。新西兰大白兔42只,玻璃体腔内注入浓度为103CFU/mL烟曲霉菌孢子悬液,48小时后进行治疗并观察。实验兔随机分为6组：A组为空白对照组(6只眼),B组为空白PLGA植入(6只眼),C组为100μg/0.1mL伏立康唑眼内注射(6只眼),D组为0.5mgVCZ-DDS植入(8只眼),E组为0.9mgVCZ-DDS植入(8只眼),F组为1.2mgVCZ-DDS植入(8只眼),B-F组均联合玻璃体切除术。术后不同时间点检测前房闪辉、房水细胞及玻璃体混浊度,抽取玻璃体腔液涂片查菌丝及真菌培养,并组织病理学检查,观察真菌及药物对眼组织的影响。另取健康新西兰大白兔6只,玻璃体切割术后植入1.2mgVCZ-DDS1枚,术后不同时间点抽取玻璃体腔液,应用高效液相色谱-质谱分析法(HPLC-MS-MS)检测伏立康唑药物浓度。 结果A、B组所有眼均发生眼内炎,并迅速发展为全眼球炎,至眼球萎缩,接种后第1周内两组差异无统计学意义(P≥0.212)。C、D、E、F组炎症反应均较A、B组轻(P≤0.046)。C组玻璃体混浊度较E、F组重(P≤0.044),前3周前房反应较E、F组重(P≤0.031)。第10、14天时,D组玻璃体浑浊度较F组轻(P=0.023),但与E组相似(P≥0.144)。术后两个月,所有炎症控制眼,眼底清晰,无复发。玻璃体腔液涂片,A、B、C组查见菌丝,真菌培养均有阳性标本,其余各组涂片及培养全部为阴性。组织病理学,治愈眼结构正常；炎症未控制眼多萎缩,球壁结构破坏。 结论玻璃体腔植入缓释系统明显优于玻璃体腔注射给药,VCZ-DDS玻璃体腔植入治疗烟曲霉菌性眼内炎安全有效,含伏立康唑1.2mg的缓释系统疗效最佳。
[Abstract]:Objective to evaluate the effect of VCZ-DDS implanted into vitreous cavity on rabbit endophthalmitis of tobacillus fumigatus, and to explore the optimal dosage and release rule of VCZ-DDS in vivo. Methods VCZ-DDS was prepared by VCZ-DDS using PLGA as carrier. Forty-two New Zealand white rabbits were injected with 103 CFU / mL aspergillus fumigatus spore suspension for 48 hours. The experimental rabbits were randomly divided into 6 groups: group A as blank control group (6 eyes), group B as blank PLGA implantation (6 eyes), group C: 100 渭 g / 0.1 mL intraocular injection of voleconazole (6 eyes), group D: 0.5 mg VCZ-DDS implantation (8 eyes), group E 0.9 mg VCZ-DDS implantation (8 eyes) and group F 1.2mgVCZ-DDS implantation. Vitrectomy combined with B-F group (8 eyes). The turbidity of anterior chamber flashes, aqueous humor cells and vitreous bodies were detected at different time points after operation. The mycelium and fungal culture were examined by vitreous cavity smears, and the effects of fungi and drugs on the ocular tissue were observed by histopathological examination. Another 6 healthy New Zealand white rabbits were implanted with 1.2 mg VCZ-DDS1 after vitrectomy. Vitreous cavity fluid was extracted at different time points after vitrectomy. The concentration of voriconazole was determined by high performance liquid chromatography-mass spectrometry (HPLC-MS-MS). Results in group A, endophthalmitis occurred in all eyes and developed rapidly into total ophthalmitis and atrophy. In the first week after inoculation, there was no significant difference between the two groups (P 鈮，

本文编号：2110967