恒定性外斜视弱侧眼外肌形态改变及MyoD、Pax7表达研究

发布时间：2018-07-09 23:04

本文选题：恒定性外斜视 + 眼外肌　；参考：《青岛大学》2011年硕士论文

【摘要】：目的：观察恒定性外斜视弱侧眼外肌形态改变及MyoD和Pax7表达变化,探讨MyoD、Pax7在恒定性外斜视发生、发展中的作用。方法：收集2009年12月至2010年9月在青岛大学医学院附属医院眼科住院手术的恒定性外斜视患者24例作为实验组,分为儿童外斜视组、成人外斜视组各12例,另选同期角膜移植供体的眼外肌10例作为成人对照组。实验组手术截取的内直肌及对照组的内直肌固定切片后行HE染色,在光镜下观察肌纤维的大小、形态、数量、走形方向等,统计肌纤维数量及肌细胞横截面积。免疫组化法测量MyoD和Pax7的平均光密度值。结果：恒定性外斜视弱侧眼外肌肌纤维数量减少,大小及形态不一,走形方向不一致,细胞间质成分增多。成人外斜视组肌纤维数量为18.77±2.91个,成人对照组为32.53±4.75个,两者差异具有统计学意义(P0.01)。而儿童外斜视组为20.81±4.27个,与成人外斜视组比较,差异无统计学意义(P0.05)。肌纤维横截面积：成人外斜视组为261.76±35.68μm2,成人对照组708.9±62.9μm2,两者差异具有统计学意义(P0.01)。而儿童外斜视组平均肌纤维横截面积为282.46±25.22μm2,与成人外斜视组比较,差异无统计学意义(P0.05)。成人外斜视组MyoD的平均光密度值(0.011±0.006)、Pax7的值(0.013±0.005)均较成人对照组(0.028±0.0008,0.027±0.002)明显减少(P0.01)。儿童外斜视组MyoD值(0.021±0.024)、Pax7的值(0.014±0.004)分别与成人外斜视组比较,差异均无统计学意义(P0.05)。结论：恒定性外斜视弱侧眼外肌肌纤维发生了萎缩、减少为主的形态学改变,以及MyoD, Pax7的表达下降,这些改变可能与共同性外斜视斜视的发生及发展有关。
[Abstract]:Aim: to observe the morphological changes of weak lateral extraocular muscles and the expression of MyoD and Pax7 in constant exotropia, and to explore the role of MyoD Pax7 in the occurrence and development of constant exotropia. Methods: from December 2009 to September 2010, 24 patients with constant exotropia who were operated on in the affiliated Hospital of Qingdao University Medical College as experimental group were divided into two groups: child exotropia group (n = 12), adult exotropia group (n = 12) and adult exotropia group (n = 12). Another 10 cases of corneal transplantation donor were selected as the adult control group. The internal rectus muscle of the experimental group and the internal rectus muscle of the control group were fixed by HE staining. The size, shape, quantity and direction of the muscle fibers were observed under light microscope, and the number of muscle fibers and the cross sectional area of muscle cells were counted. The mean optical density of MyoD and Pax7 was measured by immunohistochemical method. Results: the number of muscle fibers in the weak lateral extraocular muscle of constant exotropia was decreased, the size and shape of the muscle fibers were different, the direction of deviation was different, and the content of the interstitial cells was increased. The number of muscle fibers in adult exotropia group was 18.77 卤2.91, and that in adult control group was 32.53 卤4.75. The difference was statistically significant (P0.01). The number of children with exotropia was 20.81 卤4.27, which was not significantly different from that of adult exotropia (P0.05). The cross sectional area of muscle fiber was 261.76 卤35.68 渭 m2 in adult exotropia group and 708.9 卤62.9 渭 m2 in adult control group. The difference was statistically significant (P0.01). The mean cross sectional area of muscle fiber in children with exotropia was 282.46 卤25.22 渭 m ~ (2), which was not significantly different from that in adult exotropia group (P0.05). The mean optical density of MyoD in adult exotropia group (0.011 卤0.006) was (0.013 卤0.005) lower than that in adult control group (0.028 卤0.0008 卤0.027 卤0.002). The values of MyoD (0.021 卤0.024) and Pax7 (0.014 卤0.004) in children with exotropia were not significantly different from those in adult exotropia (P0.05). Conclusion: the weak lateral extraocular muscle fibers of the concomitant exotropia may be related to the occurrence and development of concomitant exotropia with atrophy, decreased morphologic changes, and decreased expression of MyoD, Pax7, which may be related to the occurrence and development of concomitant exotropia.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R779.6

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