S100A7在喉鳞癌组织中表达的研究

发布时间：2018-07-11 18:25

  本文选题：喉鳞癌 + S100A7　； 参考：《重庆医科大学》2013年硕士论文

【摘要】：背景与目的 肿瘤的发生发展是多基因参与、多因素影响的复杂过程。在头颈部恶性肿瘤发生过程中，上皮细胞的异常增殖被认为与肿瘤的形成紧密相关。喉鳞癌是最常见的头颈部恶性肿瘤之一。近年来喉鳞癌的发病率有逐年增加的趋势，，虽然在过去的数年里，经综合治疗（手术+放疗+化疗）喉癌患者的生存率有所提高，但晚期患者的预后仍然很差。故早期诊断对病人接受更好的治疗、延长生存时间具有重要的意义。迄今为止，尚未发现确定的生物学标志物提示潜在的肿瘤形成的恶化过程，同位素标记相对量和绝对量及液相色谱分析法显示S100A7可作为头颈部鳞状细胞癌的标志性蛋白。既往亦有研究显示S100A7在乳腺癌、肺鳞癌、食管鳞癌等多种鳞癌中过表达，S100A7蛋白在细胞增殖、信号传导、炎性反应及血管形成等病理生理过程中起着重要的作用，而关于S100A7在喉鳞癌组织中的表达情况及S100A7能否作为辅助诊断及判断预后的一个标志尚未被证实。本研究通过检测S100A7在喉鳞癌组织中的表达情况，初步探讨其与喉鳞癌发生发展的关系。 材料与方法 标本来源：所有喉鳞癌组织、喉鳞癌转移灶组织、正常喉粘膜组织均采自于重庆医科大学附属第二医院耳鼻咽喉头颈外科住院部住院患者。喉鳞癌及其转移灶诊断依据病理诊断证实。收集的标本置于-80℃冰箱保存待用。 方法：1.采用RT-PCR方法和Western-blot方法分别检测喉鳞癌组织、喉鳞癌转移灶组织、正常喉粘膜组织及不同病理分化程度的喉鳞癌组织中S100A7基因及蛋白表达情况；2.采用统计软件SPSS19.0对数据进行统计分析，以a=0.05为检验标准，P0.05表示在统计学上该差异具有统计学意义。 结果 1.经RT-PCR检测到喉鳞癌组织、喉鳞癌转移灶组织、正常喉粘膜组织中均有S100A7基因的表达，且在喉鳞癌组织中高表达，差异具有统计学意义（P0.05）；S100A7基因转录水平在不同病理分化程度的标本中不一样，其表达量在高分化、中分化、低分化喉鳞癌组织中依次递增，差异具有统计学意义（P0.05）。 2.Western-blot检测到喉鳞癌组织、喉鳞癌转移灶组织、正常喉粘膜组织中均有S100A7蛋白的表达，且在喉鳞癌组织中高表达，差异具有统计学意义（P0.05）；S100A7蛋白在高分化、中分化、低分化喉鳞癌组织中的表达量依次递增，差异具有统计学意义（P0.05）。 结论 1. S100A7在喉鳞癌中高表达，在喉鳞癌原发灶及转移灶中表达量高于正常喉粘膜组织。S100A7可能与喉鳞癌的发生、转移有关。 2.喉鳞癌组织中，S100A7的表达量与病理分化程度有关，S100A7可能参与了喉鳞癌的分化进展过程。
[Abstract]:Background and objective tumorigenesis and development is a complex process in which multiple genes are involved and many factors are involved. The abnormal proliferation of epithelial cells is thought to be closely related to the formation of malignant tumors in head and neck. Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant tumors in head and neck. In recent years, the incidence of laryngeal squamous cell carcinoma (LSCC) has been increasing year by year. Although the survival rate of patients with LSCC has increased in the past few years, the prognosis of advanced patients is still very poor. Therefore, early diagnosis is of great significance for patients to receive better treatment and prolong their survival time. Up to now, no identified biomarkers have been found to indicate the potential progression of tumorigenesis. The relative and absolute amounts of isotopic markers and liquid chromatography showed that S100A7 could be used as the iconic protein of squamous cell carcinoma of the head and neck. Previous studies have shown that the overexpression of S100A7 protein plays an important role in cell proliferation, signal transduction, inflammatory response and angiogenesis in breast cancer, lung squamous cell carcinoma, esophageal squamous cell carcinoma and other squamous cell carcinomas. The expression of S100A7 in laryngeal squamous cell carcinoma (LSCC) and whether S100A7 can be used as a marker for diagnosis and prognosis have not been confirmed. In this study, the expression of S100A7 in laryngeal squamous cell carcinoma (LSCC) was detected to explore the relationship between S100A7 expression and laryngeal squamous cell carcinoma (LSCC). Materials and methods: all laryngeal squamous cell carcinoma tissues, metastatic laryngeal squamous cell carcinoma tissues and normal laryngeal mucosa were collected from inpatients in the department of otolaryngology and head and neck surgery of the second affiliated Hospital of Chongqing Medical University. The diagnosis of laryngeal squamous cell carcinoma and its metastasis was confirmed by pathological diagnosis. The collected specimens were stored in-80 鈩

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