喉癌手术前后游离K-ras基因突变检测及临床意义
发布时间：2018-07-14 13:18
【摘要】： 目的:探讨喉癌患者手术前、术后第1天、术后第5天、术后第10天外周血游离K-ras基因的突变情况,及与喉癌患者TNM临床分类、临床分期、病理分级等临床因素之间的关系,为喉癌患者术前进行无创性检查,鉴别良恶性及监测预后提供理论依据,进而为喉癌的早期诊断及临床治疗提供理论依据。 方法:采集29例喉癌患者外周血,包括手术前、术后第1天、术后第5天、术后第10天外周血。取10例正常自愿献血者的外周血作为正常对照。应用液相芯片和突变富集PCR技术检测每份标本中K-ras基因12、13位编码子的点突变情况。 结果:游离DNA K-ras基因突变率,喉癌术前组与正常对照组比较,术后组与正常对照组比较,术前组与术后组比较,无显著差异(P 0.05);随喉癌的TNM临床分类、临床分期、病理分级的上升有增高趋势,各临床因素内比较无显著差异(Fisher精确概率检验值0.05). 结论:游离DNA K-ras基因的点突变检测尚不能为喉癌提供临床指导;Luminex液相芯片技术及游离DNA检测在喉癌临床中有一定的应用前景。
[Abstract]:Objective: to investigate the mutation of K-ras gene in peripheral blood of laryngeal cancer patients before operation, 1 day after operation, 5 days after operation and 10 days after operation, and the relationship between the mutation of K-ras gene and clinical classification, clinical stage, pathological grade and other clinical factors in patients with laryngeal carcinoma. It provides theoretical basis for preoperative noninvasive examination, differential diagnosis of benign and malignant tumor and monitoring prognosis, and then provides theoretical basis for early diagnosis and clinical treatment of laryngeal carcinoma. Methods: the peripheral blood of 29 patients with laryngeal carcinoma was collected, including the peripheral blood before operation, 1 day after operation, 5 days after operation and 10 days after operation. The peripheral blood of 10 normal voluntary blood donors was taken as normal control. The point mutation of K-ras gene 1213 in each sample was detected by liquid phase microarray and mutagenic enrichment PCR. Results: there was no significant difference in the mutation rate of free DNA K-ras gene between preoperative group and normal control group (P 0.05), and with the TNM clinical classification and clinical stage of laryngeal carcinoma, there was no significant difference between preoperative group and normal control group (P 0.05). The pathological grade increased with no significant difference among the clinical factors (Fisher accurate probability test 0.05). Conclusion: the point mutation detection of free DNA K-ras gene can not provide clinical guidance for laryngeal carcinoma. Luminex liquid-phase microarray technique and free DNA detection have a certain application prospect in laryngeal carcinoma.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65
本文编号：2121775
[Abstract]:Objective: to investigate the mutation of K-ras gene in peripheral blood of laryngeal cancer patients before operation, 1 day after operation, 5 days after operation and 10 days after operation, and the relationship between the mutation of K-ras gene and clinical classification, clinical stage, pathological grade and other clinical factors in patients with laryngeal carcinoma. It provides theoretical basis for preoperative noninvasive examination, differential diagnosis of benign and malignant tumor and monitoring prognosis, and then provides theoretical basis for early diagnosis and clinical treatment of laryngeal carcinoma. Methods: the peripheral blood of 29 patients with laryngeal carcinoma was collected, including the peripheral blood before operation, 1 day after operation, 5 days after operation and 10 days after operation. The peripheral blood of 10 normal voluntary blood donors was taken as normal control. The point mutation of K-ras gene 1213 in each sample was detected by liquid phase microarray and mutagenic enrichment PCR. Results: there was no significant difference in the mutation rate of free DNA K-ras gene between preoperative group and normal control group (P 0.05), and with the TNM clinical classification and clinical stage of laryngeal carcinoma, there was no significant difference between preoperative group and normal control group (P 0.05). The pathological grade increased with no significant difference among the clinical factors (Fisher accurate probability test 0.05). Conclusion: the point mutation detection of free DNA K-ras gene can not provide clinical guidance for laryngeal carcinoma. Luminex liquid-phase microarray technique and free DNA detection have a certain application prospect in laryngeal carcinoma.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.65
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