硫酸软骨素在长期甘油冷冻保存角膜中对内皮细胞活性的影响

发布时间：2018-07-17 21:06

【摘要】：目的探讨硫酸软骨素对长期甘油冷冻保存角膜内皮细胞(CEC)保护作用的机制,并与透明质酸钠进行比较,改进角膜长期保存方法。方法 60只Wistar大鼠角膜片随机分为A、B、C、D4组,A组不保存,后3组经不同方法保存2个月。所有经保存角膜片均行CEC台盼蓝-茜素红活性染色及HE染色检查。取40只SD大鼠与保存后角膜片建立Wistar-SD同种异体穿透性角膜移植模型,裂隙灯显微镜观察角膜排斥反应,测定术后不同时间点植片内TNF-α和IFN-γ表达水平,检验保存效果。结果染色结果显示,B组与C、D组比较,差异有显著性(P0.05)。B组保存效果和角膜移植术后效果均优于C组(P0.05),且植片中TNF-α和IFN-γ的表达水平低于C组和D组(P0.05),接近A组水平(P0.05)。C、D组间TNF-α、IFN-γ水平差异有显著性(P0.05)。结论甘油冷冻能够保持CEC的活性,硫酸软骨素和透明质酸钠能减少甘油冷冻直接保存时对CEC造成的损害,且硫酸软骨素能进一步增进保存效果。
[Abstract]:Objective to investigate the protective mechanism of chondroitin sulfate on long-term glycerol cryopreservation of corneal endothelial cells (CEC) and compare it with sodium hyaluronate to improve the long-term preservation of cornea. Methods 60 Wistar rats were randomly divided into group A and group A, and the latter three groups were preserved by different methods for 2 months. CEC trypan blue-alizarin red staining and HE staining were performed on all preserved corneal slices. The corneal allograft model was established in 40 SD rats and preserved corneal slices. Corneal rejection was observed by slit lamp microscope. The expression levels of TNF- 伪 and IFN- 纬 in grafts were measured at different time points after operation, and the preservation effect was examined. Results the staining results showed that group B was compared with group C D. There was significant difference between group B (P0.05), group B (P0.05) and group C (P0.05), and the expression level of TNF- 伪 and IFN- 纬 in grafts was lower than that in group C and group D (P0.05), and the level of TNF- 伪 IFN- 纬 in group A was close to group A (P0.05). There was a significant difference in the level of IFN- 纬 between group C and group C (P0.05). Conclusion Chondroitin sulfate and sodium hyaluronate can reduce the damage to CEC caused by glycerol cryopreservation, and chondroitin sulfate can further improve the preservation effect.
【作者单位】：辽宁医学院附属第一医院眼科;
【基金】：辽宁省教育厅科研基金资助项目(编号:2009A445)
【分类号】：R772.2

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