醋酸去氨加压素对豚鼠耳蜗水通道蛋白2表达的调节作用

发布时间：2018-07-26 12:48

【摘要】：目的观察醋酸去氨加压素(deamino arginine vasopressin,DDAVP)对内淋巴积水模型豚鼠耳蜗水通道蛋白2(aquaporins 2,AQP2)表达的调节,探讨梅尼埃病的发病机制。方法 40只成年豚鼠随机分为实验组和对照组,每组各20只。实验组豚鼠腹腔注射DDAVP 4μg·kg-1·d-1,共7天,构建内淋巴积水模型,对照组豚鼠腹腔注射等量生理盐水。停药7天后分别取两组各5只豚鼠耳蜗切片行HE染色,计算中阶与中阶加前庭阶横截面积比值平均值(R值)评估内淋巴积水程度,同时,2组各15只豚鼠分别采用免疫组化法和蛋白印迹法检测耳蜗AQP2蛋白的表达。结果实验组和对照组的R值分别为0.42±0.04和0.31±0.03,实验组豚鼠内淋巴积水程度重于对照组(P0.05);实验组和对照组豚鼠耳蜗AQP2均表达于血管纹、螺旋神经节细胞,其中,实验组耳蜗AQP2蛋白半定量分析(0.9954±0.0498)高于对照组(0.7664±0.1452),差异有统计学意义(P0.05)。结论DDAVP上调豚鼠耳蜗中与水转运密切相关的AQP2的基因表达,参与内淋巴积水形成。
[Abstract]:Objective to investigate the regulation of (deamino arginine vasopressin DDAVP on the expression of aquaporins 2 aquaporin-2 (AQP2) in the cochlea of guinea pigs with endolymphatic hydrops, and to explore the pathogenesis of Meniere's disease. Methods 40 adult guinea pigs were randomly divided into experimental group and control group with 20 in each group. The model of endolymphatic hydrops was established in the experimental group by intraperitoneal injection of DDAVP 4 渭 g kg-1 d -1 for 7 days, and the control group was injected with the same amount of normal saline intraperitoneally. Seven days after withdrawal, the cochlea sections of 5 guinea pigs in each group were stained with HE, and the mean ratio of cross sectional area (R value) of middle and middle order plus vestibular order was calculated to evaluate the degree of endolymphatic hydrops. The expression of AQP2 protein in cochlea was detected by immunohistochemistry and Western blotting in 15 guinea pigs in each group. Results the R values of the experimental group and the control group were 0.42 卤0.04 and 0.31 卤0.03, respectively. The degree of endolymphatic hydrops in the experimental group was more serious than that in the control group (P0.05). The cochlear AQP2 was expressed in stria vascularis and spiral ganglion cells in the experimental and control groups. The semi-quantitative analysis of AQP2 protein in cochlea of experimental group (0.9954 卤0.0498) was higher than that of control group (0.7664 卤0.1452), the difference was statistically significant (P0.05). Conclusion DDAVP up-regulates the expression of AQP2 related to water transport in guinea pig cochlea and participates in endolymphatic hydrops formation.
【作者单位】：重庆医科大学附属第一医院耳鼻咽喉科;
【基金】：国家临床重点专科建设项目(卫办医政函[2012]649号) 重庆市卫生局医学科研计划项目(2009-2-366)联合资助
【分类号】：R764.33

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