木黄酮对人喉癌细胞Hep-2诱导凋亡及抑制增殖的研究

发布时间：2018-07-27 11:56

【摘要】：目的:探讨木黄酮对人喉癌细胞Hep-2的诱导凋亡及抑制作用。方法:采用CCK-8法测定木黄酮抑制细胞系Hep-2增殖的IC50值;应用流式细胞术检测木黄酮对喉癌细胞所引起的周期分布变化与凋亡率;倒置相差显微镜下观察木黄酮干预人喉癌细胞Hep-2前后的细胞形态学变化。结果:木黄酮抑制Hep-2细胞增殖的IC50值为23.64μg/ml;采用浓度为12μg/ml和24μg/ml的木黄酮分别作用于喉癌细胞24h的凋亡率为22.4%±1.65%和30.64%±2.95%,与对照组比较差异均有统计学意义(P0.05);采用浓度为12μg/ml和24μg/ml的木黄酮分别作用于喉癌细胞48h的凋亡率为30.55%±0.72%和48.69%±1.06%,与对照组比较差异均有统计学意义(P0.05)。同一浓度的木黄酮作用于喉癌细胞24、48h的凋亡率差异有统计学意义(P0.05)。结论:木黄酮能够明显抑制人喉癌细胞Hep-2细胞增殖,并引起Hep-2细胞的凋亡,凋亡率随时间的延长和剂量的增加而增高。
[Abstract]:Objective: to investigate the apoptosis and inhibition effect of genistein on human laryngeal carcinoma cell line Hep-2. Methods: CCK-8 assay was used to determine the IC50 value of genistein inhibiting the proliferation of Hep-2 cell line, flow cytometry was used to detect the cycle distribution and apoptosis rate induced by genistein in laryngeal carcinoma cells. The morphologic changes of human laryngeal carcinoma cells before and after genistein treatment with Hep-2 were observed under inverted phase contrast microscope. Results: the IC50 value of genistein inhibiting Hep-2 cell proliferation was 23.64 渭 g / ml, and the apoptotic rate of laryngeal cancer cells treated with 12 渭 g/ml and 24 渭 g/ml for 24 h was 22.4% 卤1.65% and 30.64% 卤2.95%, respectively, which was significantly different from that of control group (P0.05), and 12 渭 g/ml at the concentration of 12 渭 g/ml. The apoptosis rates were 30.55% 卤0.72% and 48.69% 卤1.06in laryngeal carcinoma cells treated with 24 渭 g/ml genistein for 48 h, respectively, and there was significant difference compared with the control group (P0.05). The apoptosis rate of laryngeal cancer cells treated with the same concentration of genistein for 48 hours was significantly different (P0.05). Conclusion: genistein can significantly inhibit the proliferation of human laryngeal carcinoma cell Hep-2 cells and induce apoptosis of Hep-2 cells. The apoptotic rate increases with time and dose.
【作者单位】：中国人民武装警察部队后勤学院附属医院耳鼻咽喉科;天津市第五中心医院耳鼻咽喉科;
【分类号】：R739.65

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