亚砷酸联合顺铂抑制鼻咽癌细胞和逆转RASSF1A甲基化状态的作用

发布时间：2018-08-02 19:57

【摘要】：目的:研究亚砷酸(As2O3)及联合顺铂(DDP)作用于人鼻咽癌裸鼠移植瘤后对RASSF1A表达的影响。方法:建立人鼻咽癌CNE2细胞裸鼠移植瘤模型,随机分为NaCl组、As2O3组、DDP组和As2O3加DDP联合组,对标本分别采用RT-PCR法检测RASSF1AmRNA的表达;免疫组织化学SP法检测RASSF1A蛋白的表达;HRM法检测RASSF1A启动子区甲基化率。结果:各实验组均能抑制人鼻咽癌CNE2裸鼠移植瘤生长,并能明显上调抑癌基因RASSF1A的表达,各实验组RASSF1A的甲基化率明显低于NaCl组,且As2O3与DDP联合优于As2O3、DDP单一用药。结论:As2O3抑制人鼻咽癌CNE2裸鼠移植瘤的生长,促进RASSF1A的表达。As2O3可抑制鼻咽癌细胞恶性表型和逆转RASSF1A的甲基化状态。
[Abstract]:Aim: to study the effect of arsenite (As2O3) and cisplatin (DDP) on the expression of RASSF1A in nude mice transplanted with human nasopharyngeal carcinoma (NPC). Methods: the transplanted tumor model of human nasopharyngeal carcinoma (NPC) CNE2 cells in nude mice was established and randomly divided into NaCl group (as _ 2O _ 3) group and As2O3 + DDP group. The expression of RASSF1AmRNA was detected by RT-PCR method. Immunohistochemical SP method was used to detect the expression of RASSF1A protein. HRM method was used to detect the methylation rate of RASSF1A promoter. Results: all experimental groups could inhibit the growth of human nasopharyngeal carcinoma (NPC) CNE2 xenografts and up-regulate the expression of tumor suppressor gene RASSF1A. The methylation rate of RASSF1A in each experimental group was significantly lower than that in NaCl group, and the combination of As2O3 and DDP was superior to as _ 2O _ 3-dDP alone. Conclusion as _ 2O _ 3 can inhibit the growth of human nasopharyngeal carcinoma (NPC) CNE2 xenografts, promote the expression of RASSF1A. As2O3 can inhibit the malignant phenotype of nasopharyngeal carcinoma cells and reverse the methylation of RASSF1A.
【作者单位】：广东医学院附属医院耳鼻咽喉-头颈外科;广东医学院第二临床学院耳鼻咽喉科;
【基金】：广东省科技计划项目湛江市科技攻关计划项目(No:2011C3109010) 广东医学院面上培育项目(No:XK1449)
【分类号】：R739.63

【共引文献】