喉鳞状细胞癌及癌前病变的HPV感染亚型分析及临床意义

发布时间：2018-08-06 08:24

【摘要】：目的:探讨喉鳞状细胞癌和癌前病变与HPV感染亚型的相关性及可能的临床关系。方法:取31例喉鳞状细胞癌、52例癌前病变(29例声带白斑和23例喉乳头状瘤)患者的组织石蜡包埋标本切片,采用核酸分子水平的快速导流杂交基因芯片分型技术,进行37种HPV亚型检测和分型;以36例声带息肉组织作为对照组。结果:喉鳞状细胞癌组中HPV总阳性率为19.4%(6/31),声带白斑组中HPV总阳性率为0,喉乳头状瘤组中HPV总阳性率为65.2%(15/23),对照组均为阴性。HPV感染阳性的喉鳞状细胞癌标本中检出的HPV病毒均为高危型HPV16亚型;HPV感染阳性的喉乳头状瘤标本中共检出HPV病毒有6个HPV亚型分布(3例HPV16,2例HPV45,4例HPV52,1例HPV18,8例HPV6,1例HPV11);个体有2种或多种亚型混合感染。HPV感染与喉鳞状细胞癌及癌前病变患者的性别及其高、低危亚型分布差异无明显统计学意义。结论:喉鳞状细胞癌和癌前病变的发生与HPV感染相关,但与高、低危亚型分布无明显相关性;HPV检测对临床筛查喉癌和癌前病变及HPV特定亚型疫苗的研制具有积极意义。
[Abstract]:Objective: to investigate the relationship between laryngeal squamous cell carcinoma (LSCC) and precancerous lesions and subtypes of HPV infection. Methods: tissue paraffin embedded specimens of 52 patients with precancerous lesions (29 vocal cord leukoplakia and 23 laryngeal papilloma) were collected from 31 laryngeal squamous cell carcinoma (LSCC) patients. 37 HPV subtypes were detected and classified, 36 vocal cord polyps were used as control group. Results: the total positive rate of HPV was 19.4% (6 / 31) in laryngeal squamous cell carcinoma group, 0 in vocal cord leukoplakia group and 65.2% (15 / 23) in laryngeal papilloma group. In laryngeal papilloma specimens with high risk HPV16 subtype infection, 6 HPV subtypes were detected (3 HPV165, 2 HPV45, 4 HPV521, HPV521, 1 HPV6, 1 HPV11), and 2 or more subtypes of HPV were detected. The sex of patients with laryngeal squamous cell carcinoma and precancerous lesions is extremely high. There was no significant difference in the distribution of low risk subtypes. Conclusion: the occurrence of laryngeal squamous cell carcinoma and precancerous lesions is related to HPV infection, but it has no significant correlation with the distribution of high and low risk subtypes. The detection of HPVs has positive significance for clinical screening of laryngeal carcinoma and precancerous lesions and the development of specific subtypes of HPV vaccine.
【作者单位】：宁夏医科大学临床学院耳鼻咽喉头颈外科;上海市浦东新区公利医院;第二军医大学上海浦东公利临床医学院耳鼻咽喉科;复旦大学附属华山医院耳鼻咽喉科;宁夏医科大学临床学院临床病理科;
【基金】：上海市卫生局科研项目(No:20134074) 上海市科学技术委员会科研计划项目(No:I4DZI942008)
【分类号】：R739.65

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