地塞米松和西比灵对庆大霉素耳毒性的保护作用

发布时间：2018-08-07 11:12

【摘要】： 目的探讨地塞米松和西比灵对庆大霉素耳毒性的保护作用。方法40只豚鼠随机分为5组,庆大霉素组(G组)每日肌注庆大霉素150mg/kg,腹部皮下注射生理盐水0.5m1。保护组：DF组每日肌注庆大霉素150mg/kg,腹部皮下注射地塞米松3mg/kg,西比灵3mg/kg灌胃；D组每日肌注庆大霉素150mg/kg,腹部皮下注射地塞米松3mg/kg,F组每日肌注庆大霉素150mg/kg,西比灵3mg/kg灌胃。对照组每日肌注生理盐水3ml/kg,腹部皮下注射生理盐水0.5ml；每组均连续用药14天。每组动物用药前1天行听性脑干反应(ABR)检查,停药后1天行听性脑干反应(ABR)检查和耳蜗基底膜组织学观察,比较各组间ABR各波潜伏期及波间期、苏木素染色后毛细胞形态、TUNEL染色凋亡阳性毛细胞计数。结果①用药前各组动物ABR波间期及潜伏期差别无统计学意义(P0.05)。停药后庆大霉素组和保护组的各波潜伏期和波间期均较对照组延长(P0.05),保护组中除D组外,DF组和F组各波潜伏期和波间期时间较庆大霉素组短,而且DF组的时间又较F组短(P0.05)。②耳蜗基底膜苏木素染色观察：庆大霉素组和D组外毛细胞排列紊乱,大量缺失；保护组中DF组和F组外毛细胞排列不规则,少量细胞缺失；对照组耳蜗外毛细胞排列整齐,无细胞缺失。③耳蜗基底膜TUNEL染色凋亡阳性毛细胞计数：庆大霉素组和保护组较对照组明显增多,而保护组中DF组和F组凋亡细胞数较庆大霉素组和D组少(P0.05)。结论庆大霉素具有耳毒性,可导致豚鼠耳蜗毛细胞凋亡；西比灵或西比灵和地塞米松联合用药可减少庆大霉素诱导的耳蜗毛细胞凋亡,对听力有保护作用,联合用药效果优于单药应用；单独使用小剂量地塞米松对庆大霉素耳毒性没有保护作用。
[Abstract]:Objective to investigate the protective effects of dexamethasone and sibiline on gentamicin ototoxicity. Methods Forty guinea pigs were randomly divided into 5 groups. Gentamycin group (G group) was intramuscularly injected with gentamicin 150 mg / kg daily, and normal saline 0.5 mg / kg was injected subcutaneously into abdomen. In the protection group, gentamicin was injected intramuscularly (150 mg / kg), dexamethasone was injected subcutaneously into the abdomen (3 mg / kg), the group D was given gentamicin 150 mg / kg by intramuscular injection, and the control group was subcutaneously injected with dexamethasone 3 mg / kg / kg F by intramuscular injection of gentamicin 150 mg / kg per day and siberelin 3mg/kg by intragastric perfusion. The control group was intramuscularly injected with saline (3 ml / kg) and subcutaneously injected with saline (0.5 ml) in each group for 14 days. The auditory brainstem response (ABR) was examined one day before treatment in each group, and the auditory brainstem response (ABR) and cochlear basement membrane histology were observed on the 1st day after withdrawal. The latencies and interwave periods of ABR waves were compared between each group. After hematoxylin staining, the number of apoptosis-positive hair cells was detected by Tunel staining. Results 1 there was no significant difference in ABR wave interval and latency before treatment (P0.05). The latencies and intervals of each wave in gentamicin group and protection group were longer than those in control group (P0.05). The latency and time of wave interval in DF group and F group were shorter than those in gentamicin group except D group (P0.05). Moreover, the time of hair cells in DF group was shorter than that in F group (P0.05). 2. Observation of hematoxylin staining on basal membrane of cochlea: the outer hair cells in gentamicin group and D group were disordered and a large number of hair cells were absent, while in DF group and F group, the arrangement of outer hair cells in DF group and F group was irregular and a few cells were absent. In the control group, the outer hair cells of the cochlea were arranged neatly, and the number of apoptosis-positive hair cells in the basal membrane of the cochlea without cell deletion was significantly increased in the gentamicin group and the protective group compared with the control group. The number of apoptotic cells in DF group and F group was lower than that in gentamicin group and D group (P0.05). Conclusion Gentamycin has ototoxicity and can induce apoptosis of cochlear hair cells in guinea pigs, sibelium or cibezole combined with dexamethasone can reduce the apoptosis of cochlear hair cells induced by gentamicin, and has protective effect on hearing. Low dose dexamethasone alone had no protective effect on gentamicin ototoxicity.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R764.5

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