CK-19在声门上型喉癌组织中的表达及其与肿瘤深部侵袭的相关研究
发布时间：2018-08-11 20:10
【摘要】：目的：通过对39例声门上型喉癌及深部不同距离手术切缘CK-19表达的研究，为临床确定不同分期声门上型喉癌手术的深部安全切缘提供依据。 方法：收集临床资料完整的声门上型喉癌标本39例，作纵向每3mm距离连续切片，取肿瘤最大面的组织块连续切片，分别采用PV6001免疫组织化学两步法及RT-PCR方法检测39例声门上型喉癌组织及不同距离深部切缘组织中CK-19的表达及CK-19mRNA转录情况。 结果：(1)TI-T2期声门上型喉癌：CK-19表达癌组织与0.2cm、0.5cm、1cm深部切缘比较均有显著性差异(p0.05)；0.2cm、0.5cm、1cm切缘三者之间无显著性差异(p0.05)。(2)T3期声门上型喉癌：CK-19表达癌组织与0.2cm切缘表达无显著性差异(p0.05)；0.5cm和1cm无显著性差异(p0.05)；癌组织与0.5cm，0.2cm与0.5cm有显著性差异(p0.05)。(3)T4期声门上型喉癌：CK-19表达癌组织与0.2cm、0.5cm表达均无显著性差异(p0.05)；癌组织和1cm有显著性差异(p0.05)；0.2cm与1cm、0.5cm与1cm之间均有显著性差异(p0.05)。 结论：声门上喉癌原发灶T分期越晚，肿瘤黏膜下侵袭程度越重。T1-T2期声门上型喉癌，肿瘤深部2mm处CK-19表达与肿瘤组有明显差异，手术切缘至少保证2mm以上；T3期声门上型喉癌，肿瘤深部5mm处CK-19表达与肿瘤组差异明显，手术切缘应至少保证5mm；T4期声门上型喉癌，肿瘤深部5mm处CK-19表达与肿瘤组无明显差异，10mm处与肿瘤组有差异，手术切缘应选择5mm至10mm范围。
[Abstract]:Objective: to study the expression of CK-19 in 39 patients with supraglottic laryngocarcinoma and deep resection margin in order to provide evidence for clinical determination of deep safe margin of supraglottic laryngeal carcinoma at different stages. Methods: 39 cases of supraglottic laryngeal carcinoma with complete clinical data were collected. The tissue mass of the largest surface of the tumor was sectioned continuously by longitudinal section per 3mm distance. The expression of CK-19 and CK-19mRNA transcription in 39 cases of supraglottic laryngeal carcinoma tissues and deep incised margin tissues were detected by PV6001 immunohistochemical two-step method and RT-PCR method respectively. Results: (1) there was no significant difference between the expression of CK-19 and the deep incisal margin of 0.2cm ~ (0.5) cm ~ (-1) in TI-T2 stage supraglottic carcinoma (p0.05). There was no significant difference among the three groups (p0.05). (_ 2). There was no significant difference between the expression of CK-19 and 0.2cm (p0.05). There was no significant difference between 0.5cm and 1cm (p0.05), and there was no significant difference between cancer tissue and 0.5 cm ~ 0.2cm and 0.5cm (p0.05). (_ 3). There was no significant difference in the expression of CK-19 and 0.2cm ~ (0.5 cm) between cancer tissues and 1cm (p0.05) in stage T4 supraglottic laryngocarcinoma (p0.05). There was a significant difference between 0.2cm and 1 cm, 0.5 cm and 1cm (p0.05). Conclusion: the later the T stage of supraglottic carcinoma is, the more severe the submucosal invasion is. T1-T2 stage supraglottic laryngeal carcinoma. The expression of CK-19 in the deep part of the tumor is significantly different from that in the tumor group, and the surgical margin at least ensures the supraglottic laryngeal carcinoma above 2mm stage T3. The expression of CK-19 in the deep 5mm of the tumor was significantly different from that in the tumor group. The operative margin should ensure at least 5mm T4 supraglottic laryngeal carcinoma. There was no significant difference in the expression of CK-19 between the deep 5mm of the tumor and the tumor group at 10mm and that in the tumor group. The range of 5mm to 10mm should be selected at the surgical margin.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.65
本文编号：2178109
[Abstract]:Objective: to study the expression of CK-19 in 39 patients with supraglottic laryngocarcinoma and deep resection margin in order to provide evidence for clinical determination of deep safe margin of supraglottic laryngeal carcinoma at different stages. Methods: 39 cases of supraglottic laryngeal carcinoma with complete clinical data were collected. The tissue mass of the largest surface of the tumor was sectioned continuously by longitudinal section per 3mm distance. The expression of CK-19 and CK-19mRNA transcription in 39 cases of supraglottic laryngeal carcinoma tissues and deep incised margin tissues were detected by PV6001 immunohistochemical two-step method and RT-PCR method respectively. Results: (1) there was no significant difference between the expression of CK-19 and the deep incisal margin of 0.2cm ~ (0.5) cm ~ (-1) in TI-T2 stage supraglottic carcinoma (p0.05). There was no significant difference among the three groups (p0.05). (_ 2). There was no significant difference between the expression of CK-19 and 0.2cm (p0.05). There was no significant difference between 0.5cm and 1cm (p0.05), and there was no significant difference between cancer tissue and 0.5 cm ~ 0.2cm and 0.5cm (p0.05). (_ 3). There was no significant difference in the expression of CK-19 and 0.2cm ~ (0.5 cm) between cancer tissues and 1cm (p0.05) in stage T4 supraglottic laryngocarcinoma (p0.05). There was a significant difference between 0.2cm and 1 cm, 0.5 cm and 1cm (p0.05). Conclusion: the later the T stage of supraglottic carcinoma is, the more severe the submucosal invasion is. T1-T2 stage supraglottic laryngeal carcinoma. The expression of CK-19 in the deep part of the tumor is significantly different from that in the tumor group, and the surgical margin at least ensures the supraglottic laryngeal carcinoma above 2mm stage T3. The expression of CK-19 in the deep 5mm of the tumor was significantly different from that in the tumor group. The operative margin should ensure at least 5mm T4 supraglottic laryngeal carcinoma. There was no significant difference in the expression of CK-19 between the deep 5mm of the tumor and the tumor group at 10mm and that in the tumor group. The range of 5mm to 10mm should be selected at the surgical margin.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.65
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