兔棘阿米巴角膜炎模型血清抗体效价分析

发布时间：2018-08-13 17:49

【摘要】：10只健康新西兰白兔随机分为实验组(n=8)和阴性对照组(n=2)。实验组兔左眼为实验眼,右眼为空白对照眼。实验组采用氢化可的松点眼3 d后行角膜基质内注入赫氏棘阿米巴滋养体和包囊悬液(1×106/ml)0.2 ml。对照组兔角膜基质内注射等量生理盐水。于注射后每天记录兔眼角膜病变情况,刮取角膜组织进行病原学检测。于感染后不同时间采血和摘取角膜进行抗体效价检测和病理学检查。经角膜刮片镜检和角膜病理切片HE染色观察证实,实验眼感染棘阿米巴,并出现棘阿米巴角膜炎典型表现和病理改变。血清抗体效价随感染时间的延长而升高,于感染后第28天达峰值(A450值为2.2358),而后逐渐下降,并维持稳定,直到处死仍高于阴性对照组。阴性对照组血清抗体效价无明显变化。
[Abstract]:Ten healthy New Zealand white rabbits were randomly divided into two groups: experimental group (n = 8) and negative control group (n = 2). In the experimental group, the left eye was experimental eye and the right eye was blank control eye. In the experimental group, hydrocortisone was used for 3 days and the corneal stroma was injected into the trophozoites of Echinoamoeba Hersoni and the capsule suspension (1 脳 106/ml) 0.2 ml. The control group was injected with the same amount of saline into the cornea stroma. The pathological changes of rabbit cornea were recorded every day after injection and the corneal tissue was scraped for etiological examination. Blood and cornea were collected at different time after infection for antibody titer detection and pathological examination. The experimental eyes were infected with Acanthamoeba and had typical manifestations and pathological changes of Acanthamoeba keratitis. The titer of serum antibody increased with the prolongation of infection time and reached the peak value (A450 = 2.2358) on the 28th day after infection, and then decreased gradually, and remained stable until the death was still higher than that of the negative control group. The titer of serum antibody in negative control group did not change significantly.
【作者单位】：吉林医药学院;吉林市中心医院;
【基金】：吉林省教育厅课题(No.2014373) 国家自然科学基金(No.81301450)~~
【分类号】：R772.21

【相似文献】